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中华临床实验室管理电子杂志

中华临床实验室管理电子杂志 ›› 2019, Vol. 07 ›› Issue (03) : 162 -164. doi: 10.3877/cma.j.issn.2095-5820.2019.03.007

所属专题： 文献；

实验研究

循环增强荧光免疫技术与化学发光免疫法检测血浆BNP的比较研究
郑专1,†(), 张宇樯1   
  1. 1. 312000　绍兴市人民医院临床检验中心
  • 收稿日期:2018-10-20 出版日期:2019-08-28
  • 通信作者: 郑专
  • 基金资助:
    浙江省医药卫生科研面上项目(2018817)

Comparative study on CEIFA and CLIA in determination of plasma BNP

Zhuan Zheng1,†(), Yuqiang Zhang1   

  1. 1. Center of Clinical Laboratory, Shaoxing People′s Hospital, Shaoxing 312000, China
  • Received:2018-10-20 Published:2019-08-28
  • Corresponding author: Zhuan Zheng
  • About author:
    Corresponding author: Zheng Zhuan, Email:
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郑专, 张宇樯. 循环增强荧光免疫技术与化学发光免疫法检测血浆BNP的比较研究[J]. 中华临床实验室管理电子杂志, 2019, 07(03): 162-164.

Zhuan Zheng, Yuqiang Zhang. Comparative study on CEIFA and CLIA in determination of plasma BNP[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2019, 07(03): 162-164.

目的

分析和评价循环增强荧光免疫技术(cyclic enhanced immunofluorescent assay，CEIFA)与化学发光免疫法(chemiluminescent immunoassay，CLIA)在血浆B型脑钠肽(B-type natriuretic peptide，BNP)检测中的应用价值。

方法

采用CEIFA和CLIA法分别检测50例心内科患者血浆BNP的水平，对检测结果的相关性、回收率、精密度、样本稳定性进行评估。

结果

与CLIA法比较，CEIFA法回归方程为Y=0.975X＋2.910 (r=0.987，P<0.01)，回收率为94.49%~100.53%，批内和批间变异系数(CV)分别为2.85%~6.66%和6.21%~12.52%，高值和中值样本结果的稳定性更高。

结论

CEIFA法检测BNP具有较好的性能，较CLIA法有一定的优势，可满足临床试验室需求。

关键词: 循环增强荧光免疫技术, 化学发光免疫法, B型脑钠肽
Objective

To analyze and evaluate the application value of cyclic enhanced immunofluorescent assay (CEIFA) and chemiluminescent immunoassay (CLIA) in detection of plasma B-type natriuretic peptide (BNP).

Methods

The plasma BNP levels were measured by CEIFA and CLIA in 50 patients with cardiology. The correlation, recovery rate, precision and sample stability of the results were evaluated.

Results

Compared with CLIA, the regression equation of CEIFA was Y=0.975X+ 2.910 (r=0.987, P<0.01). The results of high and median samples were more stable. The recovery rate of CEIFA was 94.49%~100.53%, the intra-batch and inter-batch coefficient of variation (CV) were 2.85%~6.66% and 6.21%~12.52%. The stability of high value or median value samples was higher than that detected by CLIA.

Conclusion

CEIFA for plasma BNP detection has a good performance, and has some advantages compared with CLIA, which can meet the needs of clinical laboratories.

Key words: Cyclic enhanced immunofluorescent assay, Chemiluminescent immunoassay, B-type natriuretic peptide
图1 CEIFA和CLIA法检测50份样本BNP浓度结果的回归分析
表1 CEIFA和CLIA法检测BNP的回收试验结果
检测方法 标准液浓度(ng/L) 加入标准液前样本浓度(ng/L) 加入标准液后样本浓度(ng/L) 回收率(%)
CEIFA 75 170.6 146.8 94.49
2500 170.6 718.5 100.53
CLIA 75 178.2 152.6 95.56
2500 178.2 730.9 101.07
表2 样本稳定性试验(ng/L)
时间(h) 样本1 样本2 样本3
CEIFA CLIA CEIFA CLIA CEIFA CLIA
0 3856.3 3921.0 312.1 308.3 85.3 80.2
1 3836.1 3790.6 309.1 296 83.6 79.3
2 3810.9 3712.4 305.5 290 82.8 78.5
3 3806.3 3589.7 304.3 281 83.0 75.6
4 3794.8 3418.8 301.5 276 81.9 75.3
5 3761.3 3305.6 296.8 273 80.8 74.6
6 3715.2 3247.5 293.5 269.9 79.6 70.9
均值 3797.3* 3555.1 303.3* 284.9 82.4 76.3
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