2019年以来，新型冠状病毒肺炎（COVID-19）在世界范围内流行爆发，新型冠状病毒（SARS-CoV-2）持续变异，不仅对国际社会造成了恐慌，还对我国境外输入防控造成了巨大压力。云南地处边陲，大规模核酸筛查已成为常态化疫情防控的重要内容。在此背景下如何结合自身实际情况让SARS-CoV-2核酸检测高质量高效率进行是心血管专科医院面临的重大问题，本文依据云南省阜外心血管病医院3万多人次的SARS-CoV-2核酸检测工作，从两方面进行经验总结，一是在大量的急危重症手术情况下，如何应用快速核酸检测技术及时准确的出具报告；二是面临常规大规模筛查时如何能在保证质量的基础上，尽可能的缩短报告时间。希望可以为急诊实验室管理提供可借鉴之处。

Since 2019, the worldwide outbreak of COVID-19 and the continuous mutation of the Novel Coronavirus have not only caused panic in the international community, but also put great pressure on the prevention and control of imported COVID-19 from abroad. As Yunnan is located in a remote area, large-scale nucleic acid screening has become an important part of regular epidemic prevention and control. In this background, how to combine the actual situation of their own coronavirus nucleic acid testing with high quality and efficiency is the major issues faced by the cardiovascular hospital. Based on the SARS-CoV-2 nucleic acid test conducted by more than 30000 person-times in Fuwai Cardiovascular Hospital of Yunnan Province, this paper summarizes the experience from two aspects: first, how to use rapid nucleic acid testing technology to issue reports timely and accurately in the case of a large number of acute and critical operations; Second, how to shorten the reporting time as much as possible on the basis of ensuring the quality of routine large-scale screening.