Testing methods in clinical laboratory for Ebola virus

doi:10.3877/cma.j.issn.2095-5820.03.004

Abstract

Abstract:

Outbreaks of infection by Ebola virus (EBOV) occurred in West Africa caused the hemorrhagic diseases with high lethality in 2014. Till now, there is not any specific vaccine or treatment for this disease; early and rapid diagnosis of EBOV infection can significantly reduce the mortality. Therefore, establishment of rapid methods for detecting EBOV infection in clinical laboratories is very important in those countries with or without epidemic situation. Here we reviewed the recent progress of testing methods in clinical laboratory for Ebola virus infection.

Key words: Ebola virus, Nucleic acid, Serology, Immunohistochemistry

Xiaowei MA, Min LI. Testing methods in clinical laboratory for Ebola virus[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2014, 02(03): 148-151.

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References 32

1	World Health Organization. Ebola haemorrhagic fever [DB/OL].[2012-09] http://www.who.int/mediacentre/factsheets/fs103/en/
2	Peters CJ, LeDuc JW. An introduction to Ebola: the virus and the disease [J]. J Infect Dis, 1999, 179 (Suppl): ix-xvi.
3	Leroy EM, Kumulungui B, Pourrut X, et al. Fruit bats as reservoirs of Ebola virus [J]. Nature, 2005, 438 (7068): 575-576.
4	Feldmann H, Jones S, Klenk HD, et al. Ebola virus: from discovery to vaccine [J]. Nat Rev Immunol, 2003, 3 (8): 677-685.
5	Geisbert TW, Jahrling PB. Exotic emerging viral diseases: progress and challenges [J]. Nat Med, 2004, 10(12 Suppl): 110-121.
6	Gatherer D. The 2014 Ebola virus disease outbreak in west Africa [J].J Gen Virol, 2014, 95(Pt 8): 1619-1624.
7	Bente D, Gren J, Strong JE, et al. Disease modeling for Ebola and Marburg viruses [J]. Dis Model Mech, 2009, 2(1-2): 12-17.
8	Snowden FM. Emerging and reemerging diseases: a historical perspective [J]. Immunol Rev, 2008, 225: 9-26.
9	Tambo E, Ugwu EC, Ngogang JY. Need of surveillance response systems to combat Ebola outbreaks and other emerging infectious diseases in African countries [J]. Infect Dis Poverty, 2014, 3: 29.
10	刘阳, 史子学, 马玉堃, 等. SYBR GreenⅠ荧光定量RT-PCR检测埃博拉病毒方法的建立 [J]. 病毒学报, 2012, 28(5): 567-571.
11	Huang Y, Wei H, Wang Y, et al. Rapid detection of filoviruses by realtime TaqMan polymerase chain reaction assays [J]. Virol Sin, 2012,27(5): 273-277.
12	Towner JS, Rollin PE, Bausch DG, et al. Rapid diagnosis of Ebola hemorrhagic fever by reverse transcription-PCR in an outbreak setting and assessment of patient viral load as a predictor of outcome [J]. J Virol 2004, 78(8): 4330-4341.
13	Okware SI, Omaswa FG, Zaramba S, et al. An outbreak of Ebola in Uganda [J]. Trop Med Int Health, 2002, 7(12): 1068-1075.
14	Leroy EM, Baize S, Lu CY, et al. Diagnosis of Ebola haemorrhagic fever by RT-PCR in an epidemic setting [J]. J Med Virol, 2000, 60(4):463-467.
15	夏文英, 郑夔, 黄吉城, 等. 埃博拉病毒检测基因芯片的制备初探[J]. 中国国境卫生检疫杂志, 2011, 34(3): 150-154.
16	Gire SK, Goba A, Andersen KG, et al. Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak [J/OL].Science, 2014; http://www.sciencemag.org/content/early/2014/08/27/science.1259657?cited-by=yes&legid=sci;science.1259657v1#cited-by.
17	Lucht A, Grunow R, Otterbein C, et al. Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus [J]. Med Microbiol Immunol, 2004, 193(4): 181-187.
18	Lucht A, Grunow R, Möller P, et al. Development, characterization and use of monoclonal VP40-antibodies for the detection of Ebola virus [J].J Virol Methods, 2003, 111(1): 21-28.
19	Lucht A, Formenty P, Feldmann H, et al. Development of an immunofiltration-based antigen-detection assay for rapid diagnosis of Ebola virus infection [J]. J Infect Dis, 2007, 196(Suppl 2): S184-S192.
20	Rowe AK, Bertolli J, Khan AS, et al. Clinical, virologic, and immunologic follow-up of convalescent Ebola hemorrhagic fever patients and their household contacts, Kikwit, Democratic Republic of the Congo [J]. J Infect Dis, 1999, 179(Suppl): S28-S35.
21	Baize S, Leroy EM, Georges-Courbot MC, et al. Defective humoral responses and extensive intravascular apoptosis are associated with fatal outcome in Ebola virus-infected patients [J]. Nat Med, 1999,5(4): 423-426.
22	Busico KM, Marshall KL, Ksiazek TG, et al. Prevalence of IgG antibodies to Ebola virus in individuals during an Ebola outbreak,Democratic Republic of the Congo, 1995 [J]. J Infect Dis, 1999,179(Suppl): S102-S107.
23	Ksiazek TG, West CP, Rollin PE, et al. ELISA for the detection of antibodies to Ebola viruses [J]. J Infect Dis, 1999, 179(Suppl):S192-S198.
24	Saijo M, Niikura M, Ikegami T, et al. Laboratory diagnostic systems for Ebola and Marburg hemorrhagic fevers developed with recombinant proteins [J]. Clin Vaccine Immunol, 2006, 13(4): 444-451.
25	=Pattyn S, van der Groen G, Courteille G, et al. Isolation of Marburglike virus from a case of haemorrhagic fever in Zaire [J]. Lancet, 1977,1(8011): 573-574.
26	Le Guenno B, Formenty P, Formentry P, et al. Isolation and partial characterisation of a new strain of Ebola virus [J]. Lancet, 2006,367(9513): 816.
27	Bowen ETW, Lloyd G, Harris WJ, et al. Viral hæmorrhagic fever in Southern Sudan and Northern Zaire[J]. Lancet, 1977, 1(8011): 571-573.
28	Zaki SR, Shieh WJ, Greer PW, et al. A novel immunohistochemical assay for the detection of Ebola virus in skin: implications for diagnosis, spread, and surveillance of Ebola hemorrhagic fever.Commission de Lutte contre les Epidémies à Kikwit [J]. J Infect Dis,1999, 179(Suppl): S36-S47.
29	Ikegami T1, Miranda ME, Calaor AB,et al. Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996[J]. Exp Anim. 2002 Oct, 51(5): 447-455.
30	Geisbert TW, Jahrling PB. Differentiation of filoviruses by electron microscopy [J]. Virus Res, 1995, 39 (2-3): 129-150.
31	Geisbert TW, Rhoderick JB, Jahrling PB. Rapid identification of Ebola virus and related filoviruses in fluid specimens using indirect immunoelectron microscopy [J]. J Clin Pathol, 1991, 44(6): 521-522.
32	Bharat TAM, Noda T, Riches JD, et al. Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography[J]. Proc Natl Acad Sci U S A, 2012, 109(11): 4275-4280.

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