To develop an evaluation index system of assessing data management for investigator-initiated trial (IIT), so as to provide an objective and operable basis for scientific evaluation.
Methods
Using the Delphi method, 23 experts were invited to conduct 2 rounds of correspondence to rate the importance of each proposed indicator in the evaluation system. The index system was assessed based on expert's response rate, authority coefficient and opinion coordination degree, with final modifications incorporating experts feedback.
Results
In the 2 rounds of consultation, the positive coefficient of experts were both 100%, the credibility ratio were 0.899 and 0.890 respectively, and the Kendall's W coefficient were 0.247 and 0.375, respectively. The finalized evaluation system comprises 3 first-level indicators, 14 secondlevel indicators and 52 third-level indicators.
Conclusion
The evaluation index system based on Delphi method in this study demonstrates good reliability and practicality for assessing IIT data management.
To investigate the toxic effects of rotenone on dopaminergic neurons and the protective effects and mechanisms of MCC950 against rotenone-induced neuron damage.
Methods
The rotenone-induced SN4741 Parkinson's disease neuron cell model was constructed. The effects of rotenone on the viability of SN4741 cells were detected by cell counting kit-8 method and lactate dehydrogenase release test.Propidium iodide staining was used to detect the effect of rotenone on apoptosis of SN4741 cells. The effects of rotenone on the expression of NOD-like receptor pyrin domain containing 3 (NLRP3) and its downstream inflammatory factors in SN4741 cells were detected by Western blot and ELISA. The effect of NLRP3 inhibitor MCC950 on rotenone-induced SN4741 cell inflammation and cell apoptosis was investigated.
Results
With the increase of rotenone concentration, SN4741 cell viability decreased and apoptosis increased (P＜0.01).The expressions of NLRP3, IL-1β and IL-18 in rotenone treatment group were increased compared with those in control group (P＜0.01). Rotenone plus MCC950 treatment group alleviated the decreased cell viability, increased apoptosis and increased expression of NLRP3, IL-1β and IL-18 caused by rotenone treatment group.
Conclusion
Rotenone can induce neuronal apoptosis and cell death through activation of neuroinflammation, and MCC950 attenuates rotenone-induced neuroinflammation and cell apoptosis.
To investigate the clinical infection status of ureaplasma urealyticum (UU),mycoplasma hominis (MH) and chlamydia trachomatis (CT) in the genital tract of female infertility patients in Guangzhou area.
Methods
505 cases of female infertility patients in outpatient clinics of Guangdong Women and Children Hospital were selected, including 215 cases of primary infertility patients (primary infertility group) and 290 cases of secondary infertility patients (secondary infertility group), and 60 cases of normal pregnancy and obstetrics examination in Guangdong Women and Children Hospital in the same period of time were taken as the control group, and all of the patients were tested for CT antigen and the cultivation of UU and MH, as well as drug sensitivity test.
Results
The positive rates of single infections of UU, MH and CT in patients in the infertility group were 46.1%, 8.5% and 6.5%, respectively, which were significantly higher than those in the control group (P＜0.05); the positive rate of single infections in patients in the infertility group was 61.2% (309/505), and that of mixed infections was 11.9% (60/505), and there was no statistical significance in the difference of positive rates of mixed infections when compared with those in the control group (P＞0.05). The positive rates of UU, MH and CT single infections and mixed infections were similar in the primary and secondary infertility groups, and there was no statistically significant difference in the positive rates of single infections and mixed infections between the two groups (P＞0.05); UU and UU+MH mixed infections were both more sensitive to doxycycline and cosyntropin; the difference in the positive rate of UU was statistically significant in patients of different ages (P＜0.05), with the highest UU positivity rate in women in the ≥41-year-old group, and the differences in MH and CT positivity rates were not statistically significant in all age groups (P＞0.05)
Conclusion
Positive rates of female genital tract UU, MH, and CT infections are positively correlated with the occurrence of infertility, and female patients with all types of infertility should be intensely screened for these pathogens.
To investigate the vitro antibacterial effects of platelet-rich plasma (PRP)and platelet-rich gel supernant (PGS), prepared using different methods against common burn wound pathogens: Staphylococcus epidermidis (SE), Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA).
Methods
Clinical isolates of SA, SE, and PA were collected, and PRP was collected from healthy volunteers by mechanical collection method and manual two-step centrifugation method, and part of PRP obtained by manual two-step centrifugation method was prepared into PGS. Oxford cup assay method was used to observe the inhibition zone of PRP, PGS and control group and determine its diameter.
Results
The PRP prepared by manual two-step centrifugation method demonstrated significant antibacterial activity against SA(2.164±0.354 mm inhibitory zone) and PA (3.544±0.507 mm inhibitory zone), but no obvious inhibitory zone was observed in SE group. Neither apheresis-prepared PRP nor manual PGS showed detectable inhibition zones against any tested pathogens.
Conclusions
This study demonstrated that PRP prepared by manual two-step centrifugation method has antibacterial effect on SA and PA, but has no antibacterial effect on SE. PRP prepared by mechanical collection method and PGS prepared by manual two-step centrifugation method lack measurable antimicrobial activity against these three common burn wound pathogens.
To investigate the association between CYP2C19 gene polymorphism and voriconazole valley concentration and adverse drug reactions, so as to provide evidence for clinical dose adjustment.
Methods
327 patients treated with voriconazole from January 2021 to July 2023 who were simultaneously monitored with voriconazole concentration and detected with CYP2C19 gene polymorphism at the First Affiliated Hospital of Guangzhou Medical University were retrospectively analyzed, recorded their basic information, CYP2C19 genotype, dosage, duration of administration, blood concentration and abnormal liver function. Analysis of the relationship between CYP2C19 gene polymorphism and voriconazole steady-state plasma concentration and liver function abnormalities.
Results
CYP2C19 normal metabolizer and intermediate metabolizer accounted for 43.12%, and poor metabolizer accounted for 13.76%. There are statistical differences in serum voriconazole concentration among different CYP2C19 genotype carriers (P＜0.05). However, no statistically significant association was found between CYP2C19 gene polymorphisms and adverse drug reactions(P＞0.05).
Conclusion
CYP2C19 gene polymorphism affects voriconazole metabolism in vivo, but has no statistical correlation with adverse reactions after voriconazole use.
To identify shared key genes and pathways linking primary gouty arthritis(PGA) and hyperuricemia (HUA) through bioinformatics approaches.
Methods
Disease-associated gene targets of PGA and HUA were retrieved from GeneCards, OMIM and DisGeNET databases. After deduplication and filtration, the final gene targets for two groups of diseases were obtained. Then take the intersection and import the common gene targets into the STRING database to construct a protein-protein interaction (PPI) network diagram. Download the tsv format PPI network and import it into Cytoscape 3.8.0.Hub genes were prioritized using CytoHubba plugin with topological criteria: degree/closeness/betweenness centrality above median values, yielding top 27 hub genes. Perform GO enrichment analysis and KEGG enrichment analysis on common target genes of PGA and HUA using the DAVID database.
Results
389 genes from PGA and 802 genes from HUA were selected, 150 common target genes, according to the PPI network diagram, IL-6, RELA, APP, TNF, INS, IL-1β, CXCL8, MAPK1, and TLR4 are the common key target genes of PGA and HUA, GO enrichment showed that it was mainly related to inflammatory response, immune response, etc., KEGG signaling includes NOD-like receptor signaling, Toll-like receptor signaling, nuclear factor-kappa B signaling, HIF-1 signaling and tumor necrosis factor signaling.
Conclusion
The common key genes and signaling pathways involved in PGA and HUA provide a theoretical basis for understanding the correlation between the two diseases and subsequent related research.
Bladder cancer remains the most prevalent malignant tumor in the urinary system.Current treatment strategies are primarily determined by tumor invasiveness. Non-muscle-invasive bladder cancer (NMIBC) typically undergoes transurethral resection with adjuvant intravesical therapy, while muscle-invasive urothelial carcinoma (MIBC) requires radical cystectomy. However, both approaches face significant challenges with disease recurrence. Recent advances in cancer molecular biology, immunology, and genetics have revealed the therapeutic potential of oncolytic viruses, with promising efficacy demonstrated in preclinical bladder cancer models. This emerging modality offers novel treatment opportunities for bladder cancer management. This review comprehensively examines the current research status and recent progress in oncolytic virotherapy for bladder cancer, highlighting its potential to address the limitations of conventional treatments.
To explore the application effects of the micro-lecture combined with debriefing teaching method in standardizing resident training.
Methods
This prospective study enrolled 42 residents from the standardized resident training to base for general dentistry at the First Affiliated Hospital of Guangzhou Medical University (July 2015 to July 2023). In the first year of standardizing resident training,the residents were randomly divided into an experimental group of 22 and a control group of 20. The control group used the traditional standardizing resident training model, while the experimental group applied the micro-lecture combined with debriefing teaching method. Outcomes were assessed after one year through standardized theoretical and clinical skill examinations, supplemented by comprehensive questionnaire evaluations.
Results
After one year of rotation, the experimental group significantly outperformed the control group in case analysis and skill operation assessments (P＜0.001). However, the experimental group showed more prominent abilities in problem-solving, adaptive communication, clinical reasoning ability,teamwork, and increasing opportunities for reflection (P＜0.05). According to the combined teaching satisfaction evaluation, the experimental group scored higher than the control group in terms of enhancing knowledge mastery, improving practical abilities, self-learning abilities and learning interest, as well as improving clinical thinking abilities and clinical competence, and the differences were statistically significant (all P＜0.05).
Conclusion
The micro-lecture combined with debriefing teaching method significantly improves both academic performance and core clinical competencies in general dentistry residency training. This innovative methodology warrants broader implementation in standardized residency education programs.
