To explore the design and implementation of automatic verification rules using a laboratory information system (LIS) to optimize blood cell analysis, thereby improving laboratory efficiency and accuracy.

A total of 25 692 laboratory reports were collected and analyzed, including blood cell counts, classifications, and cell morphology indicators. Automatic verification rules encompassing patient information validation, sample information verification, critical value identification, and instrument alarm information validation were designed and implemented using the built-in scripting language of the LIS.

The implementation of the automatic verification rules, which comprised 64 rules across five categories, processed 25 692 reports. Among these, 19 140 reports passed automatic verification, resulting in a pass rate of 74.50%. A total of 6552 reports failed automatic verification, triggering 11 986 rule interceptions. The automatic verification system reduced the median turnaround time (TAT) from 15 minutes to 3 minutes, decreased the error rate of reports from 0.082% (16/19 526) to 0.008% (2/25 692), and lowered patient dissatisfaction from 15.38% (10/65) to 1.19% (1/84).

The design and implementation of automatic verification rules effectively enhance laboratory efficiency and quality, significantly improving patient satisfaction. Future research should aim to further optimize these rules to address more complex clinical scenarios and explore advanced technologies to support the continued development of automated blood cell analysis.

To evaluate the analytical performance of lipid detection items using the Six Sigma model and to develop individualized internal quality control (IQC) strategies and precise improvement measures.

The WS/T 403-2024 Analytical Quality Standards for Common Clinical Chemistry Tests served as the quality goal for lipid testing. Accuracy was assessed using data from the first 2024 external quality assessment of lipid provided by the Shaanxi Provincial Clinical Laboratory Center, while precision was evaluated based on IQC data from our laboratory. The sigma metric for each lipid parameter was calculated based on these three indices and plotted on a standardized sigma performance chart. Furthermore, individualized IQC strategies and improvement measures were formulated using the Westgard Sigma Rules flowchart and by analyzing the quality goal index.

The analytical performance of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and apolipoprotein A (Apo-A) was rated as "Good", while that of apolipoprotein B (Apo-B) achieved "World Class" level. The recommended IQC strategy for TC, TG, HDL-C, LDL-C, and Apo-A were the 1_3s/2_2s/R_4s/4_1s multi-rule approach (N=4) with a batch length of 200 samples. For Apo-B, the recommended strategy was the 1_3s multi-rule (N=2) with a batch length of 1000 samples. Notably, precision deficiencies were observed in five lipid analytes, warranting prioritized improvement measures.

The Six Sigma model demonstrates significant utility in evaluating lipid analyte performance, designing IQC strategies, and implementing quality improvements, thereby enhancing laboratory testing capabilities.

To develop and evaluate the immune response levels following SARS-CoV-2 vaccination or infection, a neutralizing antibody detection method based on fluorescent immunochromatography was developed and subsequently subjected to preliminary performance assessment.

The fluorescent immunochromatographic assay employs a competitive format. The test strip is coated with fluorescent microspheres-labeled receptor-binding domain and immobilized angiotensin-converting enzyme 2 along with sheep anti-mouse antibodies. This configuration enables quantitative detection of SARS-CoV-2 neutralizing antibodies in serum or plasma through fluorescence signals.

The method quantifies neutralizing antibodies for SARS-CoV-2 at two concentrations (906 IU/ml and 1865 IU/ml), with within-batch coefficients of variation of 10.16% and 8.20%, respectively. The between-batch coefficients of variation are 10.43% and 9.48%, respectively. This method exhibits no cross-reactivity with other antibodies, indicating robust specificity. Analytical sensitivity showed a limit of detection concentration is 125 IU/ml, with an optimal measurement range spanning 125-3600 IU/ml. The total concordance rate with the pseudovirus neutralization test (pVNT) method is 87%, with a positive concordance rate of 85% and a negative concordance rate of 100%. In addition, the Kappa coefficient was 0.54 (P<0.001), indicating that the chromatography method had moderate consistency with the pVNT results.

This rapid and accurate fluorescent immunochromatographic assay provides a reliable tool for evaluating vaccine efficacy and treatment outcomes in SARS-CoV-2 patients.

To characterize the genotype distribution and hematological profiles of thalassemia carriers among women of childbearing age in Yuhuan, Zhejiang Province, thereby providing a scientific basis for thalassemia screening and prevention.

From May 1, 2021 to February 28, 2022, routine blood tests were performed on women aged 15–49 years at Yuhuan Maternal and Child Health Hospital. Individuals with hemoglobin (Hb)＜115 g/L and mean corpuscular volume (MCV)＜82 fL, or Hb＜115 g/L and mean corpuscular hemoglobin (MCH)＜27 pg, were further subjected to thalassemia gene detection and high-resolution hemoglobin electrophoresis. The hematological results of patients with different genotype abnormalities were analyzed.

A total of 2039 women of childbearing age were included. Among them, 318 subjects meeting the criteria of Hb＜115 g/L with MCV＜82 fL, or Hb＜115 g/L with MCH＜27 pg underwent thalassemia gene detection. A total of 64 thalassemia gene carriers were identified, with a detection rate of 20.13% (64/318). Among them, 53 cases carried α-thalassemia genes, predominantly SEA type and 3.7 type; 10 cases carried β-thalassemia genes, predominantly IVS-Ⅱ-654 point mutation; and 1 case carried α/β compound thalassemia gene, with the genotype of CS+IVS-Ⅱ-654.

The α-thalassemia genes in women of childbearing age in Yuhuan area are predominantly SEA type heterozygote (--/αα) and 3.7 type heterozygote (-α/αα), while the β-thalassemia gene is predominantly IVS-Ⅱ-654. Characteristic analysis based on the hematological results of patients with genetic abnormalities can provide a reference for thalassemia screening in this region.

To determine the carrier rate of spinal muscular atrophy (SMA) among women of reproductive age in the Shaoguan, Guangdong Province, and to provide prenatal diagnosis for couples where both partners are identified carriers, aiming to prevent the birth of SMA-affected infants.

A total of 2646 pregnant women were screened using fluorescent quantitative PCR technology to detect the copy number of exons 7 and 8 (E7, E8) of the survival motor neuron gene 1 (SMN1). Prenatal diagnosis was conducted for high-risk couples who were both SMA carriers, and the fetal genotype results were verified using multiplex ligation-dependent probe amplification (MLPA).

Among the 2646 pregnant women, 54 SMA carriers were identified, yielding a carrier rate of 2.04% (54/2646). The carrier genotypes were categorized as follows: 41 cases had concurrent heterozygous deletions of SMN1 gene E7 and E8, 1 case had an isolated heterozygous E7 deletion, and 12 cases had an isolated heterozygous E8 deletion. Prenatal diagnosis was performed for two couples who were both partners carried a heterozygous SMN1-E7 deletion. The results identified one fetus with a heterozygous deletion of SMN1-E7 and E8 and one fetus with a normal genotype. These findings were consistent with the MLPA verification.

The carrier rate of SMA mutations in the pregnant population is relatively high in Shaoguan region of Guangdong. It is necessary to conduct SMA carrier screening among pregnant women and to perform invasive prenatal genetic diagnosis for high-risk fetuses whose parents are both SMA carriers. This approach is of great significance for effectively preventing the birth of SMA-affected infants and reducing the incidence of birth defects.

In order to adapt to the pace of vocational education reform, the ideological and political teaching strategy in the practical training course of parasitology laboratory in colleges and universities in regions inhabited by ethnic groups is deeply explored, so as to cultivate more medical laboratory technology professionals that meet the needs of society.

A total of 684 students majoring in medical laboratory technology from grade 2018, 2019 and 2020 in Qiannan Medical College were selected as the research objects. Among them, grade 2018 was the traditional teaching group, which adopted the traditional teaching method. Grade 2019 and 2020 were the ideological and political teaching groups of the curriculum, and they have established a perfect curriculum ideological and political database, built and practiced the "six into six integration" curriculum ideological and political systems, highlighting the characteristics of medical disciplines, curriculum ideological, political leadership and times. The assessment results and teaching satisfaction of the two groups were compared.

Through the above practices, the pass rate and excellent rate of the assessment results, the proportion of students participating in skills competitions, and the rate of students upgrading to bachelor's degree in the ideological and political education teaching group were all better than those in the traditional teaching group (P<0.05). Students generally accepted the teaching method of integrating ideological and political elements into the classroom, with a satisfaction rate exceeding 95%.

The reform and practice of ideological and political teaching in the parasitology laboratory training course in colleges in regions inhabited by ethnic groups can not only effectively improve academic performance, but also realize the organic combination of parasitic knowledge dissemination, ability training, skill improvement and ideological and political goals, and improve the quality of personnel training.

Through the construction of an industry-education integration practice platform, this study achieved a seamless alignment between industrial demands and classroom teaching, providing both theoretical support and practical pathways for cultivating innovative talents in clinical chromatography-mass spectrometry with industry adaptability.

A "Five-Integration Education System" was established, encompassing dimensions such as the integration of industrial elements with academic content, vocational standards with professional criteria, and industrial resources with pedagogical resources. Additionally, a collaborative curriculum development framework between schools and enterprises was constructed.

The university-enterprise cooperative courses were successfully developed. Feedback from employers indicated strong industrial adaptability among graduates, and the curriculum was recognized as a National First-Class Course.

The industry-education integration practice platform effectively bridges education and industry. It is a key avenue for deepening university-enterprise cooperation and for cultivating high-caliber, versatile talents.

To explore and establish an innovative training model for the clinical microbiology interns, aiming to enhance instructional quality and improve students' practical competencies.

By innovating teaching models, integrating diversified practical teaching methods, incorporating humanistic ideological and political education and communication skills training, and systematically optimizing the internship mentoring process for clinical microbiology testing, a more scientific and standardized trainee cultivation system is established. The effectiveness of this new model was evaluated by comparing two intern cohorts: A control group trained using traditional methods and an innovation group trained under the new integrated model.

A new training model was successfully established, emphasizing innovation ability, practical skills, and professional development. The model significantly improved student engagement and operational standardization. The innovation group demonstrated superior performance in instructor evaluations, periodic assessments, theoretical scores, and practical exam results compared to the control group, with all differences being statistically significant (P＜0.05).

The proposed training model is practical and effective, systematically enhancing both the quality of internship education and students' comprehensive practical abilities. It offers a viable approach to cultivating clinical microbiology professionals who meet modern healthcare demands.