Detection and application of circulating tumor cells for breast cancer

doi:10.3877/cma.j.issn.2095-5820.2013.01.007

Abstract

Abstract:

Circulating tumor cell is considered to have a close relationship with tumor relapse and metastasis. In recent years, More and more clinical trials and evidences have validated its application value in the efficacy of detection, prognosis and metastatic recurrence of breast cancer, circulating tumor cell has become a hot topic in prognostic and prediction of breast cancer. This article gives a summary in the detection and its application value of circulating tumor cell in breast cancer.

Key words: Circulating tumor cell, Breast neoplasms, Prognostic

Yanlan Yao, Lunan Wang. Detection and application of circulating tumor cells for breast cancer[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2013, 01(01): 32-37.

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References 29

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方法	基本原理
分离和富集方法
磁性细胞分选	抗原抗体反应+磁场作用
膜过滤法	利用细胞大小不同进行分离
密度梯度离心法	利用细胞密度不同进行分离
分析方法
免疫细胞化学技术	标记免疫技术+形态学分析
核酸技术	检测CTC特异的某mRNA水平
流式细胞分析	标记免疫技术+单细胞多参数分析
激光扫描细胞计量技术	流式细胞技术+图像细胞仪
生物芯片	分子生物学基因探针原理

方法	基本原理
分离和富集方法
磁性细胞分选	抗原抗体反应+磁场作用
膜过滤法	利用细胞大小不同进行分离
密度梯度离心法	利用细胞密度不同进行分离
分析方法
免疫细胞化学技术	标记免疫技术+形态学分析
核酸技术	检测CTC特异的某mRNA水平
流式细胞分析	标记免疫技术+单细胞多参数分析
激光扫描细胞计量技术	流式细胞技术+图像细胞仪
生物芯片	分子生物学基因探针原理

肿瘤标志	优点	缺点	主要应用	备注
CK-19、EpCAM	灵敏度高	不够特异	早期诊断	与其他标志联合
CA-153	与临床分期有关	单独使用不能较好早期诊断	较高的预后价值，辅助治疗监测，预测术后复发	多与其他标志联合检测早期乳腺癌
HER-2/neu	与临床分期有关	不够特异	预后判断，辅助治疗药物选择
TPS	敏感度高	不够特异	早期诊断，转移性乳癌诊断，疗效监测，预后判断	与CA-153、CA-125联合检测
hMAM	特异度强	不够成熟	早期诊断

肿瘤标志	优点	缺点	主要应用	备注
CK-19、EpCAM	灵敏度高	不够特异	早期诊断	与其他标志联合
CA-153	与临床分期有关	单独使用不能较好早期诊断	较高的预后价值，辅助治疗监测，预测术后复发	多与其他标志联合检测早期乳腺癌
HER-2/neu	与临床分期有关	不够特异	预后判断，辅助治疗药物选择
TPS	敏感度高	不够特异	早期诊断，转移性乳癌诊断，疗效监测，预后判断	与CA-153、CA-125联合检测
hMAM	特异度强	不够成熟	早期诊断

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