The stability of human urine microRNA-126 and its diagnosis value for the renal injury

doi:10.3877/cma.j.issn.2095-5820.2013.01.011

Abstract

Abstract:

Objective

To analyze the stability of microRNA-126 (miRNA-126) in human urine and evaluate its possibility in diagnosis of renal injury.

Methods

The influence of incubation time, test temperature, numbers of freeze-thaw cycles, pH value and different albumin concentration on stability of microRNA-126 in the urine was evaluated by one variable Chi-Square analysis. Real-time PCR was used to compare the levels of urinary miRNA-126 between 69 healthy volunteers and 139 patients with renal injury.T test was used to compare the variance of urinary miRNA-126 levels between the healthy and patient groups.ROC curve was used to evaluate the value of urine miRNA-126 in diagnosis of renal injury.

Results

The levels of urinary miRNA-126 were 3.81±0.33, 3.78±0.32, 3.83±0.43, 3.69±0.30 when the samples were incubated at room temperature, 4℃,-20℃, -80℃ for 24 hours respectively. The concentration of urinary miRNA-126 were 3.81±0.33, 3.75±0.39, 3.66±0.33, 3.76±0.32, 3.62±0.39 when the samples were placed at room temperature for 0, 3, 6, 12, 24 h. The levels of urinary miRNA-126 were 3.75±0.29, 3.70±0.32,3.70±0.31, 3.56±0.29 when urinary specimens were subjected to different freeze-thaw cycles(0, 2, 4, 8).The levels of urinary miRNA-126 were 3.76±0.28, 3.72±0.28, 3.65±0.33 when urinary specimens were subjected to different pH values (4.5, 7.0, 9.0). The concentrations of urinary miRNA-126 in groups (0-20,40-80, 120-140 mg/L) containing different urinary albumins (3.72±0.30, 3.63±0.38, 3.70±0.50) showed no significantly change. There were no significant differences in the levels of miRNA-126 (F value of 1.840, 1.321,2.262, 0.941, 0.411, all of P＞0.05). The level of urinary miRNA-126 in patients with renal injury (4.55±0.63)was higher than that in healthy volunteers (3.75±0.39, t=6.231, P=0.002). ROC analysis revealed that miRNA-126 had an AUC of 0.779 (95%CI: 0.681-0.877, P＜0.001) and sensitivity of 61.7%, specificity of 88.9% when the cut-off value was lg2^(50-Ct)＞4.19.

Conclusion

These results indicated that miRNA-126 in urine is relatively stable in urine. It has the potential to become a new molecular biomarker for early diagnosis of renal injury.

Key words: MicroRNA-126, Urine, Stability, Renal injury

Yang Liu, Hongyan Liang, Guangqiang Gao, Xiaofeng Jiang. The stability of human urine microRNA-126 and its diagnosis value for the renal injury[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2013, 01(01): 55-60.

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Figures/Tables 6

References 28

1	Bartel DP. MicroRNAs: Target recognition and regulatoryfunctions[J].Cell, 2009, 136: 215-233.
2	Cortez MA, Calin GA. MicroRNA identification in plasma and serum:a new tool to diagnose and monitor diseases[J]. Expert Opin Biol Ther,2009, 9: 703-711.
3	Wittmann J, Jäck HM. Serum microRNAs as powerful cancer biomarkers[J]. Biochim Biophys Acta, 2010, 1806: 200-207.
4	Wang K, Zhang S, Marzolf B, et al. Circulating microRNAs potential biomarkers for drug-induced liver injury[J]. Proc Natl Acad Sci U S A,2009, 106: 4402-4407.
5	Laterza OF, Lim L, Garrett-Engele PW, et al. Plasma microRNAs as sensitive and specific biomarkers of tissue injury[J]. Clin Chem, 2009,55: 1977-1983.
6	Chen X, Ba Y, Ma L, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases[J].Cell Res, 2008, 18: 997-1006.
7	Ji X, Takahashi R, Hiura Y, et al. Plasma miR-208 as a biomarker of myocardial injury[J]. Clin Chem, 2009, 55: 1944-1949.
8	Tijsen AJ, Creemers EE, Moerland PD, et al. MiR423-5p as acirculating biomarker for heart failure[J]. Circ Res, 2010, 106: 1035-1039.
9	Fichtlscherer S, De Rosa S, Fox H, et al. Circulating microRNAs in patients with coronary artery disease[J]. Circulat Res, 2010,107: 677-684.
10	Mitchell PS, Parkin RK, Kroh EM, et al. Circulating microRNAs as stable blood-based markers for cancer detection[J]. Proc Natl Acad Sci U S A, 2008, 105: 10513-10518.
11	Weber JA, Baxter DH, Zhang S, et al. The microRNA spectrum in 12 body fluids[J]. Clin Chem, 2010, 56: 1733-1741.
12	Tzimagiorgis G, Michailidou EZ, Kritis A, et al. Recovering circulating extracellular or cell-free RNA from bodily fluids[J]. Cancer Epidemiol,2011, 35: 580-589.
13	Cortez MA, Bueso-Ramos C, Ferdin J, et al. MicroRNAs in body fluids--the mix of hormones and biomarkers[J]. Nat Rev Clin Oncol,2011, 8: 467-477.
14	Zhang L, Huang J, Yang N, et a1. microRNAs exhibithigh frequency genomic alterations in human cancer[J]. Proc Natl Acad Sci U S A,2006, 103: 9136-9141.
15	Takamochi K, Ogura T, Suzuki K, et a1. Loss of heterozygosity on chromosomes 9q and 16p in atypical adenomatous hyperplasia concomitant with adenoearcinoma of the lung[J]. Am J Pathol, 2001, 159: 1941-1948.
16	Hanke M, Hoefig K, Merz H, et al. A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer[J]. Urol Oncol, 2010, 28: 655-661.
17	Fish JE, Santoro MM, Morton SU, et a1. miR-126 regulates angiogenic signaling and vascular integrity[J]. Dev Cell, 2008, 15: 272-284.
18	Bartel DP. MicroRNAs: target recognition and regulatory functions[J].Cell, 2009, 136: 215- 233.
19	Park NJ, Zhou H, Elashoff D, et al. Salivary microRNA: discovery,characterization, and clinical utility for oral cancer detection[J]. Clin Cancer Res, 2009, 15: 5473-5477.
20	Xie Y, Todd NW, Liu Z, et al. Altered miRNA expression in sputum for diagnosis of non-small cell lung cancer[J]. Lung Cancer, 2010, 67: 170-176.
21	Wang S, Aurora AB, Johnson BA, et al. The endothelial-specific microRNA miR-126 governs vascular integrity and angiogenesis[J].Dev Cell, 2008, 15: 261-271.
22	McDonald JS, Milosevic D, Reddi HV, et al. Analysis of circulating microRNA: preanalytical and analytical challenges[J]. Clin Chem,2011, 57: 833-840.
23	Gregory PA, Bracken CP, Bert AG, et al. MicroRNAs as regulators of epithelial-mesenchymal transition[J]. Cell Cycle, 2008, 20: 3112-3118.
24	Paterson EL, Kolesnikoff N, Gregory PA, et al. The microRNA-200 family regulates epithelial to mesenchymal transition[J]. Scientific World J, 2008, 8: 901-904.
25	Bracken CP, Gregory PA, Kolesnikoff N, et al. A double-negative feedback loop between ZEB1-SIP1 and the microRNA-200 family regulates epithelial-mesenchymal transition[J]. Cancer Res, 2008, 68:7846-7854.
26	Kato M, Zhang J, Wang M, et al. MicroRNA-192 in diabetic kidney glomeruli and its function in TGF-beta-induced collagen expression via inhibition of E-box repressors[J]. Proc Natl Acad Sci U S A , 2007,104: 3432-3437.
27	Harris TA, Yamakuchi M, Ferlito M, et al. MicroRNA-126 regulates endothelial expression of vascular cell adhesion molecule 1[J]. Proc Natl Acad Sci U S A, 2008, 105: 1516-1521.
28	Liu B, Peng XC, Zheng XL, et al. MiR-126 restoration down-regulate VEGF and inhibit the growth of lung cancer cell lines in vitro and in vivo[J]. Lung Cancer, 2009, 66: 169-175.

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