Trypan blue and eosin staining technique for rapid decalcification bone sections

doi:10.3877/cma.j.issn.2095-5820.2017.03.010

Chinese Journal of Clinical Laboratory Management(Electronic Edition) ›› 2017, Vol. 05 ›› Issue (03): 171-175. doi: 10.3877/cma.j.issn.2095-5820.2017.03.010

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• Clinical Research • Previous Articles Next Articles

Trypan blue and eosin staining technique for rapid decalcification bone sections

Yang Liu¹, Shiyun Ke², Fangmei Peng², Yuying Zhai², Shan Xiao², Xiaotian Ye², Jun Chen², Xingyan Lu²^,^†()

^1. Guangdong Food and Drug Vocation-technical School, Guangzhou 510663, China
^2. School of Life Science and Biopharmacy, Guangdong Pharmaceutical University/ Guangdong Provincial Key Laboratory of Biotechnology Candidate Drug Research, Guangzhou 510006, China

Received:2017-04-30 Online:2017-08-28 Published:2017-08-28
Contact: Xingyan Lu
About author:
Corresponding author: Lu Xingyan, Email: xingyanlu2005@126.com

Abstract

Abstract:

Objective

To explore the feasibility of applying trypan blue and eosin staining (TE) technique to pathologic slices of bone tissue decalcified by 5% nitric acid.

Method

Tibias of 10 rats were decalcified by 5% nitric acid and then paraffin embedded sections were stained by hematoxylin and eosin (HE), toluidine blue (TB) or TE.

Results

The color separation of bone trabecula and bone marrow was not obvious when using HE or TB. Addtionally, bone nucleus were not stained and the boundaries were hazy. In contrast, the trabecular and the bone marrow cells were stained deep-red and purplish red respecively when using TE method. The structure of osseous lacuna and bone cells include osteocyte, osteoblast, osteoclast and bone marrow macrophage is clear.

Conclusion

TE is suitable for pathologic slices of bone tissue decalcified by 5% nitric acid.

Key words: Rapid decalcification, Hematoxylin-eosin staining, Toluidine blue staining, Trypan blue and eosin staining

Yang Liu, Shiyun Ke, Fangmei Peng, Yuying Zhai, Shan Xiao, Xiaotian Ye, Jun Chen, Xingyan Lu. Trypan blue and eosin staining technique for rapid decalcification bone sections[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2017, 05(03): 171-175.

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Figures/Tables 6

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