Relationship between ApoE gene polymorphism and Lp-PLA2 in patients with acute cerebral infarction

doi:10.3877/cma.j.issn.2095-5820.2018.03.003

Abstract

Abstract:

Objective

To investigate the distribution of ApoE gene polymorphism in patients with acute cerebral infarction (ACI) and to explore the its correlation with the expression of lipoprotein-associated phospholipase A₂ (Lp-PLA₂).

Methods

A case-control study was conducted in 112 ACI inpatients that visited the Department of Neurology, the Third People′s Hospital of Liaocheng from July 2015 to June 2017 and 59 age-matched healthy subjects were included as control. Nucleic acid probe hybridization chips were used to detect the genotypes of ApoE and serum Lp-PLA₂ was measured by the up-converting luminescence technique.

Results

The allele frequencies of ApoE E2, E3 and E4 in the control group were 21.19%, 73.73% and 5.08%, respectively. The genotype frequency of ApoE conforms to the balance of Hardy-Weinberg. The frequency of E2 allele in ACI group (6.25%) was significantly lower than that in control group (21.19%) (P<0.01). The genotypes of ε2/2 and ε2/3 in ACI group were significantly lower than those in control group (P<0.05), and the genotype of ε3/4 in ACI group was higher than that in control group (P<0.05). Significantly different levels of the serum Lp-PLA₂ were found between the two group (106.16±38.85 ng/ml,in control group and 146.39±57.90 ng/ml in, ACI group, P<0.01). There was no significant difference between the serum level of Lp-PLA₂ in the E4 carriers of the control group and in non-E4 carriers of the ACI group (P>0.05), while there were significant differences among the other groups (P<0.01). The AUC value of serum Lp-PLA₂ was 0.790 (95%CI 71.9-86.1). The critical point of clinical diagnosis is 123.01ng/ml,the sensitivity was 67.8%, the specificity was 69.6%, and the expression level of Lp-PLA₂ was significant (P<0.01) for judging the occurrence of ACI. Logistic regression analysis showed that Lp-PLA₂ and ApoE E4 alleles were both risk factors for the diagnosis and treatment of ACI.

Conclusion

The ApoE E4 allele is a risk factor for acute cerebral infarction and Lp-PLA₂ expression is closely related with ACI patients. The detection of ApoE genotype and serum Lp-PLA₂ is of certain clinical significance in the diagnosis of ACI.

Key words: Acute cerebral infarction, Apolipoprotein E, Gene polymorphism, Allele, Lipoprotein-associated phospholipase A₂

Shunfeng Zhao, Jianhong Wang, Hongxia Xu, huiying Hu, Laichun Song, Liguo Chang, Zhenwei Guo. Relationship between ApoE gene polymorphism and Lp-PLA₂ in patients with acute cerebral infarction[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2018, 06(03): 140-144.

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Figures/Tables 8

References 17

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?		对照组(n=59)	ACI组(n=112)	χ²值	P值
基因型		?	?	?	?
?	ε2/2	4(6.78%)	1(0.89%)	4.718	0.049
?	ε3/3	35(59.32%)	80(71.43%)	2.572	0.125
?	ε4/4	1(1.69%)	3(2.68%)	0.164	1.000
?	ε2/3	15(25.42%)	11(9.82%)	7.296	0.012
?	ε2/4	2(3.39%)	1(0.89%)	1.398	0.274
?	ε3/4	2(3.39%)	16(14.29%)	4.871	0.034
等位基因频率		?	?	?	?
?	E2	21.19%	6.25%	9.037	0.005
?	E3	73.73%	83.48%	2.357	0.163
?	E4	5.08%	10.27%	1.492	0.268

?		对照组(n=59)	ACI组(n=112)	χ²值	P值
基因型		?	?	?	?
?	ε2/2	4(6.78%)	1(0.89%)	4.718	0.049
?	ε3/3	35(59.32%)	80(71.43%)	2.572	0.125
?	ε4/4	1(1.69%)	3(2.68%)	0.164	1.000
?	ε2/3	15(25.42%)	11(9.82%)	7.296	0.012
?	ε2/4	2(3.39%)	1(0.89%)	1.398	0.274
?	ε3/4	2(3.39%)	16(14.29%)	4.871	0.034
等位基因频率		?	?	?	?
?	E2	21.19%	6.25%	9.037	0.005
?	E3	73.73%	83.48%	2.357	0.163
?	E4	5.08%	10.27%	1.492	0.268

组别		n	x	SD	SE	95%CI	F值	P值
对照组	E4	5	146.84	53.76	24.04	80.09-213.58	91.94	0.00
对照组	非E4	54	102.39	35.54	4.84	92.69-112.09
ACI组	E4	20	248.47	68.90	15.41	216.22-280.72
ACI组	非E4	92	124.19	17.62	1.84	120.55-127.84

组别		n	x	SD	SE	95%CI	F值	P值
对照组	E4	5	146.84	53.76	24.04	80.09-213.58	91.94	0.00
对照组	非E4	54	102.39	35.54	4.84	92.69-112.09
ACI组	E4	20	248.47	68.90	15.41	216.22-280.72
ACI组	非E4	92	124.19	17.62	1.84	120.55-127.84

组别		Mean Difference(I-J)	SE	P值	95%CI
对照组E4	ACI组E4	-101.64^*	17.17	0.000	-135.54	-67.73
	对照组非E4	44.45^*	16.05	0.006	12.75	76.14
	ACI组非E4	22.64	15.77	0.153	-8.49	53.78
ACI组E4	对照组E4	101.64^*	17.17	0.000	67.73	135.54
	对照组非E4	146.08^*	8.99	0.000	128.33	163.83
	ACI组非E4	124.28^*	8.47	0.000	107.55	141.00
对照组非E4	对照组E4	-44.45^*	16.05	0.006	-76.14	-12.75
	ACI组E4	-146.08^*	8.99	0.000	-163.83	-128.33
	ACI组非E4	-21.80^*	5.89	0.000	-33.43	-10.18
ACI组非E4	对照组E4	-22.64	15.77	0.153	-53.78	8.49
	ACI组E4	-124.28^*	8.47	0.000	-141.00	-107.55
	对照组非E4	21.80^*	5.89	0.000	10.18	33.43

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Relationship between ApoE gene polymorphism and Lp-PLA₂ in patients with acute cerebral infarction

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?	B	SE	Wald	P值	95%CI
LpPLA₂	-0.035	0.007	23.498	0.000	0.952	0.980
E4	-2.1	0.865	5.891	0.015	0.022	0.668
常量	7.635	2.325	10.786	0.001	?	?

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