To study the appropriate proportion of self-mixing and mixed quality control products on the immunofluorescence project and evaluate its stability as to ensure an effective quality control of the corresponding indirect immunofluorescence (IIF) detection project in our laboratory.

By use of the lab-collected antinuclear antibodies (ANA), antimitochondrial antibody (AMA), antikeratin antibodies (AKA) and antineutrophil cytoplasm antibodies (p-ANCA) of each individual strong positive serum, the serum was then mixed in appropriate proportion and the weakly positive 4-item composite quality control was made for testing.

AKA could not be mixed into weakly positive quality control products due to mixed interference. ANA, AMA, p-ANCA and negative serum could be configured into weakly positive quality control products according to the proportion of 1:1:1.5:3, and the stable time could be up to 20 weeks.

Not all autoantibodies are suitable for the preparation of mixed quality control products. The three weakly positive autoantibodies (ANA, AMA and p-ANCA) of the self-made fluorescent immunoassay have good stability and can be used for daily quality control in clinical laboratories.