Abstract:
Objective To observe the effects of Lyciumbarbarum polysaccharide(LBP)on the proliferation of limbal stem cells (LSCs) by isolation and culture of LSCs of rat in vitro, and to provide experimental data for the study of LSCs cultured in traditional Chinese medicine in vitro.
Methods LSCswere isolated and subcultured in vitro, which were preliminarily identified by the characteristics of cells morphology with microscope and the detection of transcription factor p63 with immunofluorescence. Assess the impact of LBP on LSCs proliferation. Control group and LBP group with 20 cases in each group. The expression of proliferating cell nuclear antigen (PCNA) was detected by Western blot.
Results LSCs observed under the microscope were small in size, polygonal or elliptical, with large nuclei and a large proportion of nucleus/cytoplasm. Immunofluorescence showed that isolation and culture of LSCs of rat in vitro could express p63 with positive rate 76.41±1.66%; the expression of PCNA in the LBP group was significantly higher than that in the control group (P<0.05).
Conclusion LSCs were isolated and cultured in vitro preliminarily, and LBP can promote and maintain the proliferation and growth of LSCs in vitro.
Key words:
Limbal stem cells,
Lyciumbarbarum polysaccharide,
Culture in vitro,
p63,
Proliferating cell nuclear antigen
Zheng Liang, Jiaqi Li, Yuhua Lv, Zhiliang Cai, Yan Chao, Houquan Zhou, Yanjun Lu. Research of effects of lyciumbarbarum polysaccharides on proliferation of the limbal stemcells in vitro[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2020, 08(04): 246-250.