Analysis of serological test results of ABO neonatal hemolytic disease

doi:10.3877/cma.j.issn.2095-5820.2022.01.001

Abstract

Abstract:

Objective

To provide laboratory evidence for clinical diagnosis and treatment,the laboratory serological test results of neonatal ABO hemolytic disease were analyzed.

Methods

From May 2017 to April 2021, 500 neonatal blood samples suspected of neonatal ABO hemolytic disease who were hospitalized in the Fifth Affiliated Hospital of Guangzhou Medical University were used microcolumn gel method for ABO and Rh blood group identification and direct anti-human globulin test (DAT), free antibody test, and erythrocyte antibody emission test for hemolysis.

Results

Among the 500 children, there were 273 positive serological tests with a positive rate of 54.6%. Among them, the positive rate of children with type A blood was higher than that of type B blood, and the difference between the two was statistically significant (P＜0.05). The positive rate of DAT was 8.8% (24/273), the positive rate of free antibody test was 77.3% (211/273), the positive rate of erythrocyte antibody emission test was 100% (273/273), the positive rate of serological test in female children was higher than that in male children, and the difference was statistically significant (P＜0.05), the positive detection rate of ABO hemolytic disease in newborns of different ages was different, and the difference was statistically significant (P＜0.05). The rate in 3 days of age was higher than that of 4 to 7 days, and those with a daily age of 4~7d are higher than the daily age＞7 d, the difference was statistically significant (P＜0.0167).

Conclusions

The three serological hemolysis tests can provide a reliable laboratory basis for the diagnosis of neonatal ABO hemolytic disease. The red blood cell antibody release test is the most valuable test among the three tests. The blood type, gender and age of the newborn have certain influence on the laboratory results.

Key words: Infant, Newborn, Diseases, Hemolysis, Serological test

Aiqun Huang, Kai Sun, Huihong Chen, Shuzhen Wei, Sicheng Ye, Liuling Deng. Analysis of serological test results of ABO neonatal hemolytic disease[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2022, 10(01): 1-5.

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References 30

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项目	阳性（例）	阴性（例）	总例数（例）	阳性率（%）
A型血患儿	137	90	227	60.4
B型血患儿	136	137	273	49.8^a
合计	273	227	500	54.6

项目	阳性（例）	阴性（例）	总例数（例）	阳性率（%）
A型血患儿	137	90	227	60.4
B型血患儿	136	137	273	49.8^a
合计	273	227	500	54.6

项目	阳性（例）	阴性（例）	总例数（例）	阳性率（%）
女患儿	142	98	240	59.2
男患儿	131	129	260	50.4^a

项目	阳性（例）	阴性（例）	总例数（例）	阳性率（%）
女患儿	142	98	240	59.2
男患儿	131	129	260	50.4^a

项目	阳性（例）	阴性（例）	总例数（例）	阳性率（%）
≤3 d	145	64	209	69.4
4~7 d	94	97	191	49.2^a
＞7 d	34	66	100	34.0^ab

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