Preliminary study on bacteriostatic-effect of Qiaoqinqingfei on Klebsiella pneumoniae and Staphylococcus aureus

doi:10.3877/cma.j.issn.2095-5820.2022.01.003

Abstract

Abstract:

Objective

Serum pharmacology was used to evaluate the bacteriostatic effect of Qiaoqinqingfei on Klebsiella pneumoniae and Staphylococcus aureus in vitro.

Methods

Rabbits were given with Qiaoqinqingfei by gavage. Blood samples were taken at 0.5 h, 1 h, 2 h, 3 h on the first day and at 2 d, 3 d, 4 d, 5 d, 6 d, 7 d, 8 d, 9 d and 10 d for 10 consecutive days, respectively. Serum containing drugs were prepared, and the antibacterial effect of Qiaoqinqingfei on Staphylococcus aureus ATCC25923 and Klebsiella pneumoniae ATCC700603 was observed by CCK-8 method.

Results

The bacteriostatic rate of Qiaoqinqingfei against Staphylococcus aureus was 97.86±0.60 at 10 day, the effective bacteriostatic rate of 7 cases＞50, which was statistically significant (P＜0.05). The bacteriostatic rate of Klebsiella pneumoniae was 39.29±6.50, and the effective bacteriostatic rate of 2 cases＞50 at 10 day, which was not statistically significant.

Conclusion

The antibacterial effect of Qiaoqinqingfei on Staphylococcus aureus was better than that of Klebsiella pneumonia.

Key words: Chinese herbal, Klebsiella pneumoniae, Staphylococcus aureus

Huiling Liang, Chao Han, Linying Zheng, Zhen Huang, Donghua Gao. Preliminary study on bacteriostatic-effect of Qiaoqinqingfei on Klebsiella pneumoniae and Staphylococcus aureus[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2022, 10(01): 13-16.

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References 29

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项目	肺炎克雷伯菌				金黄色葡萄球菌
项目	用药前	用药0.5 h	用药5 d	用药10 d		用药前	用药0.5 h	用药5 d	用药10 d
抑菌率＜50%	7	7	7	5	7		7	1	0
抑菌率＞50%	0	0	0	2	0		0	6	7
P值				0.533				0.030	0.008

项目	肺炎克雷伯菌				金黄色葡萄球菌
项目	用药前	用药0.5 h	用药5 d	用药10 d		用药前	用药0.5 h	用药5 d	用药10 d
抑菌率＜50%	7	7	7	5	7		7	1	0
抑菌率＞50%	0	0	0	2	0		0	6	7
P值				0.533				0.030	0.008

项目	肺炎克雷伯菌	金黄色葡萄球菌
用药前	0.47±1.25	0.29±1.30
用药0.5 h	13.00±3.99	18.43±3.57
用药5 d	22.29±5.25	90.71±6.97^a
用药10 d	39.29±6.50	97.86±0.60^a

项目	肺炎克雷伯菌	金黄色葡萄球菌
用药前	0.47±1.25	0.29±1.30
用药0.5 h	13.00±3.99	18.43±3.57
用药5 d	22.29±5.25	90.71±6.97^a
用药10 d	39.29±6.50	97.86±0.60^a

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