To investigate the effect of mixed cells with different concentrations of Rh CcEe on serological detection methods.

21 Rh CcEe mixed test cell samples were prepared using irregular antibody screen reagents (human red blood cells) with a 5% gradient concentration difference. Rh CcEe blood groups were detected by microcolumn agglutination method and serological test tube method, and the differences between the two methods were analyzed and compared.

There was no significant difference between the two methods in double population (DP) and mixed field (MF) results for Rh CcEe blood group samples (P＜0.05). When detecting Rh CcEe blood group samples, 10% or more of mixed cells can result in DP by microcolumn agglutination, and 5% or more of the mixed cells can result in MF by serological test tube. The MF intensity grading of serological test tube method has a certain corresponding relationship with the gradient concentration.

The DP results of microcolumn agglutination method had no intensity grading, and failed to determine the proportion of mixed cells. By simulating different concentrations of Rh CcEe mixed test cells, a standard curve for serological in MF intensity grading can be established, which helps to infer the proportion of Rh CcEe mixed cells in the sample.