Home    中文  
 
  • Search
  • lucene Search
  • Citation
  • Fig/Tab
  • Adv Search
Just Accepted  |  Current Issue  |  Archive  |  Featured Articles  |  Most Read  |  Most Download  |  Most Cited

Chinese Journal of Clinical Laboratory Management(Electronic Edition) ›› 2023, Vol. 11 ›› Issue (04): 207-213. doi: 10.3877/cma.j.issn.2095-5820.2023.04.003

• Experiment Researchs • Previous Articles     Next Articles

Comparative study on the detection ability of BacT/ALERT two blood culture systems and eight blood culture bottles for simulated bacteremia specimens

Xiaosu Wang, Zheng Dai, Jiajia Zhu, Qichao Li, Litao Zhang()   

  1. Department of Clinical Laboratory, Wuhan Asia General Hospital, Wuhan Hubei 430050, China
  • Received:2023-04-12 Online:2023-11-28 Published:2024-01-06
  • Contact: Litao Zhang

Abstract:

Objective

To compared the difference of aerobic and facultative anaerobic blood culture bottles BacT/ALERT FA Plus, FA, PF Plus, PF and SA, as well as anaerobic and facultative anaerobic bottles BacT/ALERT FN Plus, FN and SN in the detection of simulated bacteremia samples, and to verify the accuracy of monitoring blood volume with BacT/ALERT VIRTUO blood culture instrument.

Methods

23 kinds of bacteria were selected to prepare bacterial suspension. Healthy human blood and bacterial suspension were added into the bottles, and cultured in two blood culture systems respectively. The mean detection time (time-to-detect, TTD) and positive growth were compared. A certain amount of phosphate buffer saline (PBS) was injected into the blood culture bottles, and then placed in the BacT/ALERTVIRTUO blood culture instrument to get the monitoring volume, and calculate the difference between the injected volume and the monitoring volume.

Results

Except for Staphylococcus hominis and Cryptococcus neoformans, the positive detection rates of the other 21 strains were 100%. There was no significant difference in the average TTD time of the five kinds of aerobic bottles in the detection of aerobic and facultative anaerobic bacteria and fungi. For the detection of anaerobes and facultative anaerobes, SN bottle was the fastest. In the detection of aerobic and facultative anaerobes, anaerobic and facultative anaerobes, the average TTD time of BacT/ALERTVIRTUO blood culture instrument was shortened by 1.5~8 h and 3~8 h compared with BacT/ALERT 3D blood culture instrument, and the difference was statistically significant. The difference between the monitoring volume and the actual injection volume was no statistical difference.

Conclusions

There was no significant difference in the ability of five kinds of aerobic bottles to detect common bacteremia specimens. The anaerobic bottle SN is superior to FN and FN Plus bottles in shortening the detection time. BacT/ALERTVIRTUO blood culture instrument can significantly shorten the detection time of common bacteremia samples and is accurate and reliable in monitoring blood volume.

Key words: blood culture, culture medium, bacteria, bloodstream infection

京ICP 备07035254号-20
Copyright © Chinese Journal of Clinical Laboratory Management(Electronic Edition), All Rights Reserved.
Tel: 020-81341720 Fax: 020-37103505 E-mail: clinlab@cma.org.cn
Powered by Beijing Magtech Co. Ltd