Kingella: An emerging pathogen of invasive infections disease in young children

doi:10.3877/cma.j.issn.2095-5820.2024.04.006

Abstract

Abstract:

Kingella is an emerging pathogen causing pediatric osteoarthritis, septic bacteremia, infective endocarditis, meningitis and other invasive infectious diseases. It is transmitted through close contact and mostly affects infants aged 6 months～4 years. In this paper, the biological characteristics, pathogenicity, clinical characteristics, new methods of laboratory diagnosis and drug resistance of the Kingella were briefly reviewed, in order to improve the understanding of the genus by clinicians and provide important reference for the early diagnosis, treatment and prognosis of kimellosis.

Key words: Kingella, emerging pathogen, infant, invasive infections disease, antibiotics sensitivity

Lei Zhu, Qingyi Zhu. Kingella: An emerging pathogen of invasive infections disease in young children[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2024, 12(04): 229-237.

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References 663

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生物化学反应	反硝化金氏菌	产吲哚金氏菌	金氏金氏菌	口金氏菌
β-溶血	-	-	+	-
4%NaCl生长	-	+	-	ND
NO₃还原	+	-	-	-
NO₂还原	+	-	-	-
从NO₂产气	+	-	-	-
磷酸酶	-	+	+	+
消化素酶	-	+	+	ND
产吲哚	-	+	-	-
水解Tween-20	-	+	-	-
DNA G+C mol%	54～47	49	47	56～58

生物化学反应	反硝化金氏菌	产吲哚金氏菌	金氏金氏菌	口金氏菌
β-溶血	-	-	+	-
4%NaCl生长	-	+	-	ND
NO₃还原	+	-	-	-
NO₂还原	+	-	-	-
从NO₂产气	+	-	-	-
磷酸酶	-	+	+	+
消化素酶	-	+	+	ND
产吲哚	-	+	-	-
水解Tween-20	-	+	-	-
DNA G+C mol%	54～47	49	47	56～58

特征	金氏金氏菌	奈氏金氏菌	反硝化金氏菌	口金氏菌	蜜熊金氏菌
G染色形态特征	成双球杆菌或短链	长链球杆菌，早期自溶	成双球杆菌或短链	杆状或球杆状，成双或短链状	杆状
溶血	β	β	-	-	-
琼脂点蚀	+	+	+	+	-
色素	-	-	-	-	+
氧化酶	+	+	+	+	+
触酶	-	-	-	-	-
葡萄糖产酸	+	+	+	+	-
麦芽糖产酸	+	-	-	-	-
H₂S产生	-	-	-	+	-
DNA酶	-	-	-	-	+
NO₂^-还原	-	-	+	-	-
NO₃^-还原	-	-	+	-	-
磷酸酶活性	+	+	-	+	-

特征	金氏金氏菌	奈氏金氏菌	反硝化金氏菌	口金氏菌	蜜熊金氏菌
G染色形态特征	成双球杆菌或短链	长链球杆菌，早期自溶	成双球杆菌或短链	杆状或球杆状，成双或短链状	杆状
溶血	β	β	-	-	-
琼脂点蚀	+	+	+	+	-
色素	-	-	-	-	+
氧化酶	+	+	+	+	+
触酶	-	-	-	-	-
葡萄糖产酸	+	+	+	+	-
麦芽糖产酸	+	-	-	-	-
H₂S产生	-	-	-	+	-
DNA酶	-	-	-	-	+
NO₂^-还原	-	-	+	-	-
NO₃^-还原	-	-	+	-	-
磷酸酶活性	+	+	-	+	-

靶基因	引物序列（F-正向，R-反向）	探针（5′→3′）	金氏金氏菌	奈氏金氏菌
rtxA	GCGCACAAGCAGGTGTACAA，ACCTGCTGCTACTGTACCTGTTTTAG	TTGAACAAAGCTGGACACG	+	+
	TGCCAAAGTAAAACCAGCTGAA，AACTTACCTAATTTTGGCAAAGCAA	TGACAACAAACCGCTAATCATTTCTAAGGCC	+	+
rtxB	CAACATAAGCCGCCAGTTGA，ACAATTAAAGCAATGGCAGTTGAG	ATCCCAACGGCGCGTCATTTGT	+	+
groEL	GCAAGAAGTCGGCAAAGAG，GTCAAACAACAACACAAATGGG	CGCGATCGCGACAAGTAGCCACGGTCAAGATCGCG	+	-
	CACGTTCTGCATTGAAATCTG，GTTCACTACTACAGACGCTTC	CGCTGACCAAGAAGCTGGCGTG	-	+
	CCGATTTGAAACGCGGTATT，TTTGCCAACTTGCTCGTCAG	AGTGGCGGCTTTGGTTGGG	+	-
mdh	TGTTCCGCATTGCTTCTG，TCATGCCGTCCAACAATG	CATCATCACGCCCTGAACGGCTT	+	-

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