The standard WS/T 229-2024 "Physical, chemical, and morphological examination of urine" was officially promulgated in May 2024. This revision aims to align with the rapid advancements in urinalysis technology in recent years, addressing prevalent issues in clinical laboratories such as inconsistent standards, non-standardized operations, and challenges in achieving result harmonization across practical procedures, performance verification, quality control, and reporting. Its objective is to further elevate the standardization and result comparability of routine urinalysis in China. The core updates of the new standard are manifested in 3 key areas: Expansion of terminology definitions, enhancement of total process quality management, and standardization of result reporting. Notably, it introduces, for the first time, a systematic integration of requirements for "personnel, facilities, and environment" and incorporates critical quality control components such as "performance verification" and "reflex testing rules". This article provides a detailed interpretation of the standard's main content, with particular focus on the revisions. Furthermore, it offers practical application guidance and recommendations for clinical laboratories, drawing upon relevant international and domestic standards, literature, and real-world scenarios. The ultimate goal is to facilitate effective implementation of the standard in support of accurate and efficient clinical diagnosis and treatment.