Multidrug-resistant genes of mycobacterium tuberculosis and associated detection methods

doi:10.3877/cma.j.issn.2095-5820.2020.02.001

Abstract

Abstract:

Tuberculosis (TB) has been a serious threat to human health. With the continuous development of anti-TB drugs and the improvement of people’s health conditions, the incidence and mortality of TB has been greatly reduced. However, after the 1980s, the incidence of TB increased due to the emergence of drug-resistant strains and multidrug resistance of mycobacterium tuberculosis (MTB). In this paper, we review the rapid diagnosis etiology of multidrug resistant tuberculosis (MDR-TB), the types of drug-resistant genes, the mechanism of drug resistance and the new diagnostic techniques based on molecular biology .

Key words: Mycobacterium tuberculosis, Multidrug resistant genes, Molecular biological diagnosis

Qingyi Zhu. Multidrug-resistant genes of mycobacterium tuberculosis and associated detection methods[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2020, 08(02): 65-70.

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药物(Drug)		基因(Gene)	产物(Product)
第一线药(firstline)
?	异烟肼(isoniazid)	katG	过氧化氢酶-过氧化物酶-过氧亚硝基酶T
?		inhA	烟酰胺腺嘌呤二核苷酸-依赖性烯酰酰基载体蛋白还原酶
?		ndh	烟酰胺腺嘌呤二核苷酸脱氢酶
?		ahpC	烷基过氧化氢还原酶C
?	利福平(rifampin)	rpoB	DNA导向RNA聚合酶β
?	吡嗪酰胺(pyrazinamide)	pncA	吡嗪酰胺酶/烟酰胺酶
?	乙胺丁醇(ethambutol)	embCAB	膜吲哚乙酰肌醇阿拉伯糖基转移酶
?	链霉素(streptomycin)	rpsL	30S核糖体蛋白S12
?		rrs	16S rRNA
?		gidB	葡萄糖抑制有丝分裂蛋白B
第二线药(Second line)
?	阿米卡星-卡那霉素(amikacin-kanamycin)	rrs	16S rRNA
?	阿米卡星-卡那霉素(amikacin-kanamycin)	eis	增强细胞内生存蛋白
?	乙硫异烟胺(ethionamide)	ethA	单氧酶
?		inhA	NADH-依赖性烯酰酰基载体蛋白还原酶
?		ethR	TetR家族转录抑制因子
?		ndh	NADH脱氢酶
?	氟喹诺酮(fluoroquinolones)	gyrAB	DNA旋转酶
?	对氨基水杨酸(para-amino-salicylic acid)	thyA	胸苷酸合成酶
?	对氨基水杨酸(para-amino-salicylic acid)	folC	聚谷氨酸合成酶C

药物(Drug)		基因(Gene)	产物(Product)
第一线药(firstline)
?	异烟肼(isoniazid)	katG	过氧化氢酶-过氧化物酶-过氧亚硝基酶T
?		inhA	烟酰胺腺嘌呤二核苷酸-依赖性烯酰酰基载体蛋白还原酶
?		ndh	烟酰胺腺嘌呤二核苷酸脱氢酶
?		ahpC	烷基过氧化氢还原酶C
?	利福平(rifampin)	rpoB	DNA导向RNA聚合酶β
?	吡嗪酰胺(pyrazinamide)	pncA	吡嗪酰胺酶/烟酰胺酶
?	乙胺丁醇(ethambutol)	embCAB	膜吲哚乙酰肌醇阿拉伯糖基转移酶
?	链霉素(streptomycin)	rpsL	30S核糖体蛋白S12
?		rrs	16S rRNA
?		gidB	葡萄糖抑制有丝分裂蛋白B
第二线药(Second line)
?	阿米卡星-卡那霉素(amikacin-kanamycin)	rrs	16S rRNA
?	阿米卡星-卡那霉素(amikacin-kanamycin)	eis	增强细胞内生存蛋白
?	乙硫异烟胺(ethionamide)	ethA	单氧酶
?		inhA	NADH-依赖性烯酰酰基载体蛋白还原酶
?		ethR	TetR家族转录抑制因子
?		ndh	NADH脱氢酶
?	氟喹诺酮(fluoroquinolones)	gyrAB	DNA旋转酶
?	对氨基水杨酸(para-amino-salicylic acid)	thyA	胸苷酸合成酶
?	对氨基水杨酸(para-amino-salicylic acid)	folC	聚谷氨酸合成酶C

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