Establishment of reference intervals for peripheral blood NLR， PLR and LMR in the apparently healthy elderly over 60 years of age in Nanjing

doi:10.3877/cma.j.issn.2095-5820.2025.01.005

Abstract

Abstract:

Objective

To establish and verify the reference intervals of neutrophil to lymphocyte ratio （NLR）， platelet to lymphocyte ratio （PLR） and lymphocyte to monocyter ratio （LMR） in the elderly over 60 years of age in Nanjing area.

Methods

A total of 851 healthy elderly people who underwent physical examination in Lanyuan Community Health Service Center of Xuanwu District in Nanjing from January to December 2021 were selected as the research subjects by complete randomization method. There were 378 males in the male group and 473 females in the female group， aged from 60 to 89 years old. NLR， PLR and LMR were calculated according to the parameters of blood cell analysis， and the reference intervals of NLR， PLR and LMR were established and the established reference intervals were verified.

Results

NLR showed the rule of male higher than female. PLR and LMR showed the rule of female higher than male， and the differences were statistically significant （P＜0.05）. Therefore， the reference intervals for peripheral blood NLR， PLR and LMR in apparently healthy elderly over 60 years old were established according to gender：For male NLR is 0.83～3.09， PLR is 53.08～162.67， LMR is 2.95～10.42； for female NLR is 0.75～2.91， PLR is 55.55～173.57， LMR is 3.54～11.64. After verification， the set reference interval is effective.

Conclusion

The reference intervals of NLR， PLR and LMR in peripheral blood of elderly people over 60 years old in Nanjing area were established and verified， which provided a reference for the clinical application of NLR， PLR and LMR in elderly people in Nanjing area.

Key words: neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, lymphocyte to monocyte ratio, reference interval, healthy elderly over 60 years

Zhihui Xu. Establishment of reference intervals for peripheral blood NLR， PLR and LMR in the apparently healthy elderly over 60 years of age in Nanjing[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2025, 13(01): 35-40.

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References 32

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项目	男性（n=378）	女性（n=473）	P 值
NLR	1.74（1.43～2.22）	1.60（1.28～1.91）	＜0.001
PLR	96.79（80.60～119.96）	102.90（82.49～124.71）	0.041
LMR	5.34（4.28～6.75）	6.74（5.33～8.65）	0.000

项目	男性（n=378）	女性（n=473）	P 值
NLR	1.74（1.43～2.22）	1.60（1.28～1.91）	＜0.001
PLR	96.79（80.60～119.96）	102.90（82.49～124.71）	0.041
LMR	5.34（4.28～6.75）	6.74（5.33～8.65）	0.000

项目	老年男性参考个体（n=23）		老年女性参考个体（n=25）
项目	超出参考区间个数	超出比例/%	超出参考区间个数	超出比例/%
NLR	2	8.70	1	4.00
PLR	2	8.70	0	0
LMR	1	4.35	1	4.00

项目	老年男性参考个体（n=23）		老年女性参考个体（n=25）
项目	超出参考区间个数	超出比例/%	超出参考区间个数	超出比例/%
NLR	2	8.70	1	4.00
PLR	2	8.70	0	0
LMR	1	4.35	1	4.00

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