In vitro experimental study on the antibacterial effect of platelet-rich plasma prepared by different methods against common pathogenic bacteria in burn infected wound

doi:10.3877/cma.j.issn.2095-5820.2025.02.004

Abstract

Abstract:

Objective

To investigate the vitro antibacterial effects of platelet-rich plasma (PRP)and platelet-rich gel supernant (PGS), prepared using different methods against common burn wound pathogens: Staphylococcus epidermidis (SE), Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA).

Methods

Clinical isolates of SA, SE, and PA were collected, and PRP was collected from healthy volunteers by mechanical collection method and manual two-step centrifugation method, and part of PRP obtained by manual two-step centrifugation method was prepared into PGS. Oxford cup assay method was used to observe the inhibition zone of PRP, PGS and control group and determine its diameter.

Results

The PRP prepared by manual two-step centrifugation method demonstrated significant antibacterial activity against SA(2.164±0.354 mm inhibitory zone) and PA (3.544±0.507 mm inhibitory zone), but no obvious inhibitory zone was observed in SE group. Neither apheresis-prepared PRP nor manual PGS showed detectable inhibition zones against any tested pathogens.

Conclusions

This study demonstrated that PRP prepared by manual two-step centrifugation method has antibacterial effect on SA and PA, but has no antibacterial effect on SE. PRP prepared by mechanical collection method and PGS prepared by manual two-step centrifugation method lack measurable antimicrobial activity against these three common burn wound pathogens.

Key words: mechanical collection method, manual two-step centrifugation method, plateletrich plasma, burn wound, infection, antibacterial effect

Hao Huang, Qun Liang, Yang Zhao. In vitro experimental study on the antibacterial effect of platelet-rich plasma prepared by different methods against common pathogenic bacteria in burn infected wound[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2025, 13(02): 84-90.

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References 30

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项目	PLT/10⁹/L					WBC/10⁹/L
项目	A	B	C	D	均值/x̄ ± s	A	B	C	D	均值/x̄ ± s
手工法 PRP	900	924	970	995	947.3±46.54	0.48	0.54	0.60	0.69	0.578±0.078
机采法 PRP	1003	1010	989	970	993.0±17.64	0.01	0.01	0.01	0.00	0.008±0.005
P 值	-	-	-	-	0.092	-	-	-	-	<0.000 1

项目	PLT/10⁹/L					WBC/10⁹/L
项目	A	B	C	D	均值/x̄ ± s	A	B	C	D	均值/x̄ ± s
手工法 PRP	900	924	970	995	947.3±46.54	0.48	0.54	0.60	0.69	0.578±0.078
机采法 PRP	1003	1010	989	970	993.0±17.64	0.01	0.01	0.01	0.00	0.008±0.005
P 值	-	-	-	-	0.092	-	-	-	-	<0.000 1

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