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中华临床实验室管理电子杂志

中华临床实验室管理电子杂志 ›› 2018, Vol. 06 ›› Issue (04) : 222 -225. doi: 10.3877/cma.j.issn.2095-5820.2018.03.007

所属专题： 文献；

实验研究

血红素加氧酶1对人脊髓星形胶质细胞缺氧/复氧损伤的保护作用
梁飞1, 魏魏1,†(), 药泽蓉1, 李晓泽1   
  1. 1. 046000　长治市妇幼保健院医学遗传室
  • 收稿日期:2017-11-24 出版日期:2018-11-28
  • 通信作者: 魏魏
  • 基金资助:
    山西省卫生厅科研课题项目(201202001)

Protective effect of HO-1 overexpression against hypoxia-reoxygenation injury of human astrocytes of spinal cord

Fei Liang1, Wei Wei1,†(), Zerong Yao1, Xiaoze Li1   

  1. 1. Genetic Laboratory, Changzhi Maternal and Child Health Hospital, Changzhi 046000, China
  • Received:2017-11-24 Published:2018-11-28
  • Corresponding author: Wei Wei
  • About author:
    Corresponding autor: Wei Wei, Email:
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引用本文：

梁飞, 魏魏, 药泽蓉, 李晓泽. 血红素加氧酶1对人脊髓星形胶质细胞缺氧/复氧损伤的保护作用[J]. 中华临床实验室管理电子杂志, 2018, 06(04): 222-225.

Fei Liang, Wei Wei, Zerong Yao, Xiaoze Li. Protective effect of HO-1 overexpression against hypoxia-reoxygenation injury of human astrocytes of spinal cord[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2018, 06(04): 222-225.

目的

探讨血红素加氧酶1(heme oxygenase, HO-1)过表达对人脊髓星形胶质细胞(human astrocytes of spinal cord, HA-sp)缺氧/复氧损伤的保护作用。

方法

通过给予HA-sp氯化钴(250 μl/L CoCl2)刺激模拟缺氧，然后再去除CoCl2刺激，建立细胞缺氧/复氧损伤模型。采用免疫印迹(western blot, WB)方法，从蛋白水平上检测不同刺激条件下HA-sp中HO-1的表达。采用四甲基偶氮唑蓝(methylthiazolyldiphenyl-tetrazolium bromide, MTT)方法，分析单纯给予缺氧/复氧刺激后以及上调HO-1表达的同时给予缺氧/复氧刺激后HA-sp的存活率变化。

结果

缺氧/复氧刺激条件下，HA-sp中HO-1蛋白过表达。利用锌原卟啉(ZnPPIX)抑制缺氧/复氧刺激条件下HO-1过表达后，HA-sp在缺氧/复氧刺激条件下存活率明显降低(P<0.01)；与单纯给予缺氧/复氧刺激后HA-sp的存活率相比，用钴原卟啉(CoPP)上调HA-sp中HO-1表达的同时给予缺氧/复氧刺激，细胞存活率明显升高(P<0.01)。

结论

利用CoPP上调HA-sp中HO-1的表达，具有抗细胞缺氧/复氧损伤的保护作用。

关键词: 血红素加氧酶1, 人脊髓星形胶质细胞, 缺氧/复氧, 存活率
Objective

To investigate the protective effect of overexpressed heme oxygenase-1(HO-1) in hypoxia-reoxygenation of human astrocytes of spinal cord(HA-sp).

Methods

Hypoxia-reoxygenation mode was established by oxygen provision after CoCl2 stimulation. The expression of HO-1 under different stimulation was detected by Western blotting. MTT assay was used to examine the relative survival of cell under hypoxia-reoxygenation as well as after up-regulated HO-1.

Results

HO-1 was upregulated by hypoxia/re-oxygen stimulation. The relative survival rate of HA-sp under hypoxia-reoxygenation was significantly decreased after HO-1 protein overexpression was inhibited by ZnPPIX (P<0.01), while the relative survival rate of HA-sp which was pretreated by CoPP was significantly increased compared with that of cells under hypoxia-reoxygenation (P<0.01).

Conclusion

HO-1 overexpression by CoPP exerts protective effect on hypoxia-reoxygenation injury of HA-sp. The result of this study may offer new thought for the therapy of hypoxia-reoxygenation injury of human spinal cord in the future.

Key words: Heme oxygenase-1, Human astrocytes of spinal cord, Hypoxia-reoxygenation, Survival rate
图1 缺氧再复氧对HA-sp中HO-1表达的影响
图2 缺氧再复氧条件下，ZnPPIX对HA-sp中HO-1表达的影响
图3 不同浓度ZnPPIX对HA-sp存活率的影响
图4 缺氧再复氧条件下，抑制HO-1表达对HA-sp存活率的影响
图5 缺氧再复氧条件下，CoPP预处理对HO-1表达的影响
图6 缺氧/复氧条件下，上调HO-1表达对HA-sp存活率的影响
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