研究免疫荧光项目自配混合质控品的配制比例，并评价其稳定性，以确保本实验室相应间接免疫荧光法(indirect immunofluorescence，IIF)检测项目的质量控制有效。

利用本实验室采集的抗核抗体(antinuclear antibodies，ANA)、抗线粒体抗体(antimitochondrial antibody，AMA)、抗角蛋白抗体(antikeratin antibody，AKA)、抗中性粒细胞胞浆抗体(antineutrophil cytoplasm antibodies，p-ANCA)各单项强阳性血清，经实验确认后进行适当比例混合，自制弱阳性4项目复合质控品进行检测。

AKA因混合干扰无法配制成弱阳性混合质控品，ANA、AMA、p-ANCA和阴性血清可以按照1:1:1.5:3比例配置成弱阳性混合质控品，稳定时间可达20周。

并非所有自身抗体均适合配制混合质控品。自制荧光免疫方法的3项自身抗体弱阳性质控品的稳定性良好，可用于临床实验室的日常质量控制使用，既方便操作又节约成本，可供其他实验室借鉴参考。

To study the appropriate proportion of self-mixing and mixed quality control products on the immunofluorescence project and evaluate its stability as to ensure an effective quality control of the corresponding indirect immunofluorescence (IIF) detection project in our laboratory.

By use of the lab-collected antinuclear antibodies (ANA), antimitochondrial antibody (AMA), antikeratin antibodies (AKA) and antineutrophil cytoplasm antibodies (p-ANCA) of each individual strong positive serum, the serum was then mixed in appropriate proportion and the weakly positive 4-item composite quality control was made for testing.

AKA could not be mixed into weakly positive quality control products due to mixed interference. ANA, AMA, p-ANCA and negative serum could be configured into weakly positive quality control products according to the proportion of 1:1:1.5:3, and the stable time could be up to 20 weeks.

Not all autoantibodies are suitable for the preparation of mixed quality control products. The three weakly positive autoantibodies (ANA, AMA and p-ANCA) of the self-made fluorescent immunoassay have good stability and can be used for daily quality control in clinical laboratories.