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中华临床实验室管理电子杂志 ›› 2021, Vol. 09 ›› Issue (03) : 174 -181. doi: 10.3877/cma.j.issn.2095-5820.2021.03.010

综述

新型冠状病毒及其分子生物学诊断新技术
朱镭1, 朱庆义2,()   
  1. 1. 030013 山西太原,山西省儿童医院
    2. 030032 山西太原,太原金域临床检验所
  • 收稿日期:2020-11-03 出版日期:2021-08-28
  • 通信作者: 朱庆义

Novel coronavirus and molecular diagnostic technique

Lei Zhu1, Qingyi Zhu2,()   

  1. 1. Shanxi Children?s Hospital, Taiyuan Shanxi 030013, China
    2. Taiyuan KingMed Center for Clinical Laboratory, Taiyuan Shanxi 030032, China
  • Received:2020-11-03 Published:2021-08-28
  • Corresponding author: Qingyi Zhu
引用本文:

朱镭, 朱庆义. 新型冠状病毒及其分子生物学诊断新技术[J/OL]. 中华临床实验室管理电子杂志, 2021, 09(03): 174-181.

Lei Zhu, Qingyi Zhu. Novel coronavirus and molecular diagnostic technique[J/OL]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2021, 09(03): 174-181.

冠状病毒(Corona virus)为正向单链RNA病毒,目前已知引起人类疾病的冠状病毒有7种。新型冠状病毒(SARS-CoV-2)为β属冠状病毒,主要引起新型冠状病毒肺炎(COVID-19)。目前,COVID-19在世界范围内快速传播和蔓延,已成为一种常态化防控的新发传染病。本文对SARS-CoV-2的基因分型和实验室诊断新技术,包括实时荧光定量聚合酶链反应(FQ-PCR)、环介导等温扩增(LAMP),POCT免疫层析快速诊断试验,核酸质谱分析(MALDI-TOF)和基因测序等作了简要概述。

Corona virus is a plus-single-stranded RNA virus, and currently there are 7 types of coronavirus known to cause diseases in human. Novel coronavirus (SARS-CoV-2) is a β genus coronavirus and mainly causes novel coronavirus pneumonia. At present, COVID-19 is rapidly spreading around the world and has become an emerging infectious diseases under regular prevention and control. In this paper, new techniques for genotyping and laboratory diagnosis of SARS-CoV-2, including real-time fluorescent quantitative polymerase chain reaction (FQ-PCR), loop-mediated isothermal amplification (LAMP), rapid diagnostic test of POCT immunochromatography, nucleic acid mass spectrometry (MALDI-TOF) and gene sequencing, were briefly reviewed.

表1 WHO报告各机构建立PCR法检测SARS-CoV-2的基因位点
表2 WHO报告各机构采用PCR试验检测SARS-CoV-2基因位点及其引物设计
表3 ORF 1ab和N基因引物和探针序列
表4 COVID-19 RT-LAMP检测法引物序列
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