The data derived from molecular test and analysis of a person′s genetic makeup, or the genetic profile of individual′s tumor are the basis for clinic physician to proceed with precision medicine accordingly. However, to obtain accurate molecular data, it is not only relied on the advanced equipment and methods, but also requires the appropriate procedures for laboratory management and quality control. Under these circumstance, a laboratory technician with appropriate certification should be equipped with professional skills/knowledge and perform the test by following the standard of experimental procedures strictly, and then high quality data should be obtained. Thus, both complying systematically standard operating procedure and meanwhile paying serious attention to each step are critical for the accuracy and repeatability of test results. In this paper, we discussed the issues occurred in the laboratory when next generation sequencing and ARMS-PCR were conducted to draw everyone′s attention and in return to improve the quality of performance for reliable results.