Comparative study on CEIFA and CLIA in determination of plasma BNP

doi:10.3877/cma.j.issn.2095-5820.2019.03.007

Abstract

Abstract:

Objective

To analyze and evaluate the application value of cyclic enhanced immunofluorescent assay (CEIFA) and chemiluminescent immunoassay (CLIA) in detection of plasma B-type natriuretic peptide (BNP).

Methods

The plasma BNP levels were measured by CEIFA and CLIA in 50 patients with cardiology. The correlation, recovery rate, precision and sample stability of the results were evaluated.

Results

Compared with CLIA, the regression equation of CEIFA was Y=0.975X+ 2.910 (r=0.987, P<0.01). The results of high and median samples were more stable. The recovery rate of CEIFA was 94.49%~100.53%, the intra-batch and inter-batch coefficient of variation (CV) were 2.85%~6.66% and 6.21%~12.52%. The stability of high value or median value samples was higher than that detected by CLIA.

Conclusion

CEIFA for plasma BNP detection has a good performance, and has some advantages compared with CLIA, which can meet the needs of clinical laboratories.

Key words: Cyclic enhanced immunofluorescent assay, Chemiluminescent immunoassay, B-type natriuretic peptide

Zhuan Zheng, Yuqiang Zhang. Comparative study on CEIFA and CLIA in determination of plasma BNP[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2019, 07(03): 162-164.

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Figures/Tables 3

References 10

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检测方法	标准液浓度(ng/L)	加入标准液前样本浓度(ng/L)	加入标准液后样本浓度(ng/L)	回收率(%)
CEIFA	75	170.6	146.8	94.49
CEIFA	2500	170.6	718.5	100.53
CLIA	75	178.2	152.6	95.56
CLIA	2500	178.2	730.9	101.07

检测方法	标准液浓度(ng/L)	加入标准液前样本浓度(ng/L)	加入标准液后样本浓度(ng/L)	回收率(%)
CEIFA	75	170.6	146.8	94.49
CEIFA	2500	170.6	718.5	100.53
CLIA	75	178.2	152.6	95.56
CLIA	2500	178.2	730.9	101.07

时间(h)	样本1		样本2		样本3
时间(h)	CEIFA	CLIA	CEIFA	CLIA	CEIFA	CLIA
0	3856.3	3921.0	312.1	308.3	85.3	80.2
1	3836.1	3790.6	309.1	296	83.6	79.3
2	3810.9	3712.4	305.5	290	82.8	78.5
3	3806.3	3589.7	304.3	281	83.0	75.6
4	3794.8	3418.8	301.5	276	81.9	75.3
5	3761.3	3305.6	296.8	273	80.8	74.6
6	3715.2	3247.5	293.5	269.9	79.6	70.9
均值	3797.3*	3555.1	303.3*	284.9	82.4	76.3

时间(h)	样本1		样本2		样本3
时间(h)	CEIFA	CLIA	CEIFA	CLIA	CEIFA	CLIA
0	3856.3	3921.0	312.1	308.3	85.3	80.2
1	3836.1	3790.6	309.1	296	83.6	79.3
2	3810.9	3712.4	305.5	290	82.8	78.5
3	3806.3	3589.7	304.3	281	83.0	75.6
4	3794.8	3418.8	301.5	276	81.9	75.3
5	3761.3	3305.6	296.8	273	80.8	74.6
6	3715.2	3247.5	293.5	269.9	79.6	70.9
均值	3797.3*	3555.1	303.3*	284.9	82.4	76.3

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