Comparative analysis and prospect of hepatitis B virus detection methods

doi:10.3877/cma.j.issn.2095-5820.2020.01.001

Abstract

Abstract:

Hepatitis B virus (HBV) is extremely harmful and highly contagious. About one million people worldwide has been died of HBV infection every year. The virus is hard to be completely eradicated, and full remission is impossible. It is particularly important to do a good job in the early detection, early treatment of the disease and detection of HBV in the treatment process. This paper first introduces the common clinical detection methods such as colloidal gold method, enzyme-linked immunosorbent assay, time-resolved fluorescence assay, chemiluminescent immunoassay and FQ-PCR method, and makes a detailed comparative analysis of them. Then it introduces the new research direction of HBV detection. Finally, the current situation and development direction of HBV detection are discussed and prospected.

Key words: Hepatitis B virus, Enzyme-linked immunosorbent assay, Time-resolved fluorescence assay, Chemiluminescent immunoassay

Lina Ma, Juntao Zhang, Shengnan Li. Comparative analysis and prospect of hepatitis B virus detection methods[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2020, 08(01): 2-5.

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References 18

1	Ganem D. Hepatitis B virus infection-natural history and dinical consequnces[J]. Nengl J Med, 2004,1(12):1268.
2	Wright TL. Introduction chronic hepatitis B infection[J]. Am J Gastroenterol, 2006,101(supplement 1):S1-S6.
3	中华医学会感染病学分会.慢性乙型肝炎防治指南(2010年) [J]. 中国肝脏病杂志, 2011,3(1):40-56.
4	中华医学会肝病学分会，中华医学会感染病学分会，Association C M.慢性乙型肝炎防治指南(2010年版)[J].中华肝脏病杂志, 2011,19:13-14.
5	刘玉昆，黄晓霞，吴远萍，等.乙肝病毒标志物阳性产妇乳汁中乙肝病毒检测结果分析[J].中国妇幼健康研究, 2011,22(5):581.
6	邬剑，李慧玲. ELISA法和胶体金试纸条法在乙肝表面抗原检测中的应用研究[J].医学信息, 2018.9155-156.
7	徐红珍，王璐.两种方法检测乙肝血清学标志物的比较分析[J].当代医学, 2011,17(19):29-30.
8	韦志鹃.两种不同方法对乙型肝炎病毒感染血清指标检测结果的比较[J].国际检验医学杂志, 2016,37(10):1429-1431.
9	崔艳军.化学发光免疫分析法与酶联免疫吸附法检测乙肝病毒标志物临床分析[J].影响研究与医学应用, 2018,2(12):240-241.
10	冯文哲.化学发光法和酶联免疫吸附法应用于乙肝病毒血清检测的效果比较[J].临床医药文献电子杂志, 2018,5(26):149-151.
11	Su HL, Huang RD, He SQ, et al. Cloning and sequence analysis of the DHBV genome of the brown ducksin Guilin region and establishment of the quantitative method for detecting DHBV[J]. REPROD biol Endocrnol, 2013,29(2):180-184.
12	秦望森，沈立萍，张爽，等.乙肝患者HBV感染指标、病毒复制水平与基因分型的关系分析[J].中华实验和临床病毒学杂志, 2012,26(5):328.
13	Alvarado-Mora MV, Romano CM, Gornes-Gouvea MS, et al. Phylogenetic analysis of complete genome sequences of hepatitis B virus from an Afro-Colombian community:presence of HBV F3/A1recombinat strain[J].Virol J, 2012,9:244.
14	蒋栋.乙肝病毒标志物定量检测与乙肝患者肝损伤的相关性分析[J].重庆医学, 2006,35(18):1641-1642.
15	陈海雁，张桂花，罗锦彬，等.荧光定量PCR法检测乙肝病毒DNA和乙肝病毒标志物检测的临床价值分析[J].使用临床医药杂志, 2014,18(19):108-110.
16	魏颖.分析乙肝病毒DNA病毒载量与各型乙型肝炎血清标志物之间的关系[J].系统医学, 2018,3(10):14-16.
17	穆莉莉，周晓俊，叶嘉明.基于离心式微流控技术的乙肝病毒检测新方法[J].分析实验室, 2018,37(3):316-322.
18	张秀娟.基于高通量测序技术的乙肝耐药基因突变检测方法研究[D].中国人民解放军军事医学科学院.

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