Application of patient data moving averaging method in internal quality control of neonatal genetic metabolic disease screening project

doi:10.3877/cma.j.issn.2095-5820.2019.03.009

Abstract

Abstract:

Objective

To explore the application method and value of patient data moving averaging method in internal quality control of neonatal genetic metabolic diseases screening by tandem mass spectrometry.

Methods

The neonatal genetic metabolic disease screening data were collected by tandem mass spectrometry. The internal quality control of tandem mass spectrometry testing items was monitored by using conventional commercial quality control products combined with patient data moving averaging method, and its effect was evaluated.

Results

According to the rule of the patient data moving averaging method, it was found the factors that caused the failure of 20161027 batches of experiments were the pre-analysis factors, i.e. inappropriate sample preservation leading to degradation and the quality problems of the commercial quality control products used in the 20170620 batches of experiments.

Conclusions

The patient data moving averaging method can effectively help laboratory management of internal quality control. It is the main quality control method without quality control indicators in tandem mass spectrometry detection, and is a useful supplement to the traditional quality control methods.

Key words: Moving averaging method, Tandem mass spectrometry (MS/MS), Internal quality control

Dongyang Hong, Yanyun Wang, Yun Sun, Tao Jiang. Application of patient data moving averaging method in internal quality control of neonatal genetic metabolic disease screening project[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2019, 07(03): 169-172.

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Figures/Tables 4

References 13

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指标	-3SD		＋3SD	指标	-3SD		＋3SD	指标	-3SD		＋3SD
C0	13.848	18.036	22.223	C10:2	0.007	0.015	0.023	C18:1OH	0.015	0.026	0.037
C2	10.050	16.510	22.970	C12	0.075	0.096	0.117	CIT	11.030	12.402	13.773
C3	0.988	1.356	1.723	C12:1	0.065	0.090	0.115	PHE	41.881	51.200	60.514
C3DC＋C4OH	0.106	0.140	0.174	C14	0.152	0.174	0.196	MET	13.375	20.023	26.670
C4	0.131	0.184	0.236	C14:1	0.083	0.103	0.123	TYR	76.947	89.877	102.808
C4DC＋C5OH	0.150	0.165	0.181	C14:2	0.006	0.019	0.031	ALA	234.325	298.395	362.466
C5	0.054	0.082	0.110	C14OH	0.004	0.015	0.026	LEU	99.636	125.454	151.272
C5:1	0.003	0.012	0.021	C16	2.743	3.160	3.577	ARG	0.348	5.621	10.894
C5DC＋C6OH	0.073	0.119	0.165	C16:1	0.164	0.195	0.226	ORN	95.758	107.932	120.106
C6	0.017	0.035	0.054	C16:10H	0.025	0.035	0.044	GLY	417.238	486.906	556.575
C6DC	0.047	0.123	0.200	C16OH	0.010	0.021	0.032	VAL	72.754	96.062	119.370
C8	0.042	0.058	0.074	C18	0.801	0.903	1.004	PRO	131.769	156.778	181.779
C8:1	0.082	0.110	0.138	C18:1	1.205	1.400	1.595	SA	0.110	1.041	1.971
C10	0.051	0.072	0.093	C18:2	0.110	0.165	0.220	?	?	?	?
C10:1	0.048	0.071	0.093	C18OH	0.005	0.016	0.027	?	?	?	?

指标	-3SD		＋3SD	指标	-3SD		＋3SD	指标	-3SD		＋3SD
C0	13.848	18.036	22.223	C10:2	0.007	0.015	0.023	C18:1OH	0.015	0.026	0.037
C2	10.050	16.510	22.970	C12	0.075	0.096	0.117	CIT	11.030	12.402	13.773
C3	0.988	1.356	1.723	C12:1	0.065	0.090	0.115	PHE	41.881	51.200	60.514
C3DC＋C4OH	0.106	0.140	0.174	C14	0.152	0.174	0.196	MET	13.375	20.023	26.670
C4	0.131	0.184	0.236	C14:1	0.083	0.103	0.123	TYR	76.947	89.877	102.808
C4DC＋C5OH	0.150	0.165	0.181	C14:2	0.006	0.019	0.031	ALA	234.325	298.395	362.466
C5	0.054	0.082	0.110	C14OH	0.004	0.015	0.026	LEU	99.636	125.454	151.272
C5:1	0.003	0.012	0.021	C16	2.743	3.160	3.577	ARG	0.348	5.621	10.894
C5DC＋C6OH	0.073	0.119	0.165	C16:1	0.164	0.195	0.226	ORN	95.758	107.932	120.106
C6	0.017	0.035	0.054	C16:10H	0.025	0.035	0.044	GLY	417.238	486.906	556.575
C6DC	0.047	0.123	0.200	C16OH	0.010	0.021	0.032	VAL	72.754	96.062	119.370
C8	0.042	0.058	0.074	C18	0.801	0.903	1.004	PRO	131.769	156.778	181.779
C8:1	0.082	0.110	0.138	C18:1	1.205	1.400	1.595	SA	0.110	1.041	1.971
C10	0.051	0.072	0.093	C18:2	0.110	0.165	0.220	?	?	?	?
C10:1	0.048	0.071	0.093	C18OH	0.005	0.016	0.027	?	?	?	?

质控指标	低浓度质控均值(μmol/L)	样本数据均值(μmol/L)
ALA	826.00	298.40
VAL	483.00	96.06
CIT	95.00	12.40
GLY	986.00	486.91
MET	123.00	20.02
LEU	439.00	125.45
PHE	237.00	51.20
TYR	389.00	89.88
PRO	637.00	156.78
SA	6.51	1.04
C5DC＋C4OH	0.63	0.12
C0	109.00	18.04
C2	61.40	16.51
C3	11.50	1.36
C4	2.77	0.18
C5	1.20	0.08
C6	0.56	0.04
C8	0.71	0.06
C10	0.98	0.07
C12	1.80	0.10
C14	1.87	0.17
C16	12.30	3.16
C18	2.19	0.90

质控指标	低浓度质控均值(μmol/L)	样本数据均值(μmol/L)
ALA	826.00	298.40
VAL	483.00	96.06
CIT	95.00	12.40
GLY	986.00	486.91
MET	123.00	20.02
LEU	439.00	125.45
PHE	237.00	51.20
TYR	389.00	89.88
PRO	637.00	156.78
SA	6.51	1.04
C5DC＋C4OH	0.63	0.12
C0	109.00	18.04
C2	61.40	16.51
C3	11.50	1.36
C4	2.77	0.18
C5	1.20	0.08
C6	0.56	0.04
C8	0.71	0.06
C10	0.98	0.07
C12	1.80	0.10
C14	1.87	0.17
C16	12.30	3.16
C18	2.19	0.90

[1]	Ying Tao, Yan Yang, Xiaoguang Li, Yan Han. 16731 patient data used in internal quality control of newborn screening [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2020, 08(03): 175-179.
[2]	Feng Li, Yinfeng Wang, Zhiyun Shi, Lixin Wang, Jie Cui, Jun Wei. Use the standard deviation index and coefficient of variation ratio to evaluate the consistency of different results on the application of different blood cells analyzer in the laboratory [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2016, 04(01): 37-42.

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