To explore the structure and diversity of fecal bacterial community in Fmr1 knockout mice.

10 mouse feces samples were sequenced and analyzed by 16S rRNA high-throughput sequencing.

A total of 325 280 high quality sequences were obtained from the two groups of samples. And 3 core bacteria were Bacteroidetes, Firmicutes and Proteobacteria.The analysis of Anosim showed that there were significant differences between the knockout (KO) and the wild-type (WT) groups.The repeatability of the samples within the group meets the data analysis requirements.The analysis of Adonis showed that the reliability of this test was high (P<0.05). LDA showed significant differences in intestinal microflora between KO and WT groups.Based on the distance of Unifrac,the analysis of Amova showed significant differences between KO and WT groups (P<0.05).

The intestinal microbial community structure and diversity of KO and WT groups exists significant differences,indicating that autism is closely related to the intestinal micro ecology system. The intestinal microecology system may affect the development of autism by microbial metabolism indirectly, but the exact mechanism still needs further research.