Correlational study between drug resistance and resistance genes of Enterococcus faecalis in urinary tract infection

doi:10.3877/cma.j.issn.2095-5820.2021.02.001

Abstract

Abstract:

Objective

To study the relationship between bacterial drug resistance and resistance genes of Enterococcus faecalis isolated from urinary tract infection, so as to provide the prevention and treatment basis for Enterococcus faecalis infection.

Methods

38 strains of Enterococcus faecalis with clinical urinary tract infection were collected and analyzed for drug sensitivity, theⅠ class integrase genes and drug resistant genes (qacE delta 1 - sull gene, tetM, ermB, aac (6′)/aph (2′,′), aph3′Ⅲ, ant (6) Ⅰ, VanA, VanB) were detected. PCR products of positive samples were sequenced and analyzed, and the carrying status of drug-resistant genes and phenotypes were analyzed.

Results

The drug resistance rate of 38 strains of Enterococcus faecalis to 12 antibiotics was from high to low: E(100%, 38/38), TE (94.74%, 36/38), CIP (47.37%, 18/38), LVX (47.37%, 18/38), MXF (44.74%, 17/38), HLS (31.58%, 12/38), FM (5.26%, 2/38), AMP (5.26%, 2/38), P (5.26%, 2/38), LNZ (2.63%, 1/38), VA (0%), TEC (0%); The positive rate of drug-resistant genes ranged from high to low: the positive rate of tetM gene was 92.11% (35/38), the positive rate of qacE △ 1-sull gene was 78.95% (31/38), the positive rate of aph3′Ⅲ gene was 71.05% (27/38), aac (6′)/aph (2″) gene positive rate was 44.74% (17/38), ant (6) I gene positive rate was 34.21% (13/38), and 38 strains were not detected ermB gene, VanA gene, VanB gene and Ⅰ class integron enzyme gene.

Conclusions

The drug resistance rate of 38 strains of Enterococcus faecalis to 12 antibiotics was from high to low: E (100%, 38/38), TE (94.74%, 36/38), CIP (47.37%, 18/38), LVX (47.37%, 18/38), MXF (44.74%, 17/38), HLS (31.58%, 12/38), FM (5.26%, 2/38),AMP (5.26%, 2/38), P (5.26%, 2/38), LNZ (2.63%, 1/38), VA (0%), TEC (0%); The positive rate of drug-resistant genes ranged from high to low: the positive rate of tetM gene was 92.11% (35/38), the positive rate of qacE △ 1-sull gene was 78.95% (31/38), the positive rate of aph3′Ⅲ gene was 71.05% (27/38), aac (6′)/aph (2″) gene positive rate was 44.74% (17/38), ant (6) Ⅰ gene positive rate was 34.21% (13/38), and 38 strains were not detected ermB gene, VanA gene, VanB gene and Ⅰ class integron enzyme gene.

Key words: Enterococcus faecalis, Drug resistance genes, Drug resistance, Integron

Xinglian Mai, Siyao Liu, Xuan Chen, Qianjun He, Zhongwen Zhang, Guoquan Zhong, Weihong Wen, Lingqing Xu. Correlational study between drug resistance and resistance genes of Enterococcus faecalis in urinary tract infection[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2021, 09(02): 65-70.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhlcsysgldzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-5820.2021.02.001

https://zhlcsysgldzzz.cma-cmc.com.cn/EN/Y2021/V09/I02/65

Figures/Tables 9

References 20

1	中华人民共和国国家卫生和计划生育委员会. WS/T 489-2016 尿路感染临床微生物实验室诊断[S/OL]. 北京: 中华人民共和国国家卫生和计划生育委员会官网, 2016. URL
2	陈文凤, 叶彤, 李琳, 等. 整合子在细菌耐药中的研究进展[J]. 吉林医药学院学报, 2019, 40(3):216-218.
3	沈承澜, 管军, 陶伟民, 等. 2013年至2017年上海市同仁医院重症医学科分离菌分布及耐药性分析[J]. 中国医师杂志, 2019, 21(4):548-551.
4	Sobhan N, John J, Mayrer C, et al. Gram-positive bacteria are a major reservoir of Class 1 antibiotic resistance integrons in poultry litter[J]. PNAS, 2004, 101(18):7118-7122.
5	Tan SC, Chong CW, Teh CSJ, et al. Occurrence of virulent multidrug-resistant Enterococcus faecalis and Enterococcus faecium in the pigs, farmers and farm environments in Malaysia[J]. PeerJ, 2018, 6:53.
6	Prado ACC, De Barros PP, Santos JDD, et al. Enterococcus faecium and Enterococcus faecalis in endodontic infections: antibiotic resistance profile and susceptibility to photodynamic therapy[J]. LD Science, 2017, 1:2-4.
7	Jaimee G, Halami P M. Conjugal transfer of aac(6′)Ie-aph(2″)Ia gene from native species and mechanism of regulation and cross resistance in Enterococcus faecalis MCC3063 by real time-PCR[J]. Microb Pathog, 2017, 110:546-553.
8	Kamelia O, Rava Z T, Jihan B, et al. Vancomycin and florfenicol resistant Enterococcus faecalis and Enterococcus faecium isolated from human urine in an Egyptian urban-rural community[J]. Actatropica, 2019, 201:105-209.
9	宋育明, 杨色娟, 刘小六. 某院2014-2016年泌尿道感染病原菌的耐药性分析[J]. 中国药物滥用防治杂志, 2018, 24(1):26-30.
10	胡志东, 李金, 刘郁, 等. 临床感染患者分离的肠球菌属耐药性及耐药基因的研究[J]. 中华医院感染学杂志, 2008(8):1057-1060.
11	陆金金, 刘海浪, 朱伟, 等. 2012年~2015年男性住院病人尿路感染病原菌的分布及其耐药性分析[J]. 临床外科杂志, 2019, 27(2):113-116.
12	杨俊, 李淦洪, 李乾伟, 等. 泌尿系结石患者尿路感染病原菌分布及耐药性特点分析[J]. 吉林医学, 2019, 40(7):1482-1483.
13	高杰英, 王缚鲲, 顾江, 等. 粪肠球菌和屎肠球菌耐药性分析[J]. 解放军医药杂志, 2019, 31(11):37-42.
14	欧阳娟, 阳军, 黄骥. 粪肠球菌与屎肠球菌的耐药性分析[J]. 中华临床医师杂志(电子版), 2017, 11(3):530-531.
15	施宏, 陈书韵, 杨狄, 等. 浙江地区临床分离粪肠球菌的多位点序列分型及耐药性分析[J]. 现代检验医学杂志, 2019, 34(1):29-33.
16	徐丹, 李耘, 朱赛楠, 等‍. 2004-2014年尿标本分离粪肠球菌和屎肠球菌药物敏感性变迁[J]. 中国感染控制杂志, 2017, 16(4):322-325.
17	徐韫健, 梁权辉, 刘长连. 氨基糖苷类高水平耐药肠球菌的耐药及分子机制的研究[J]. 中国实验诊断学, 2013, 17(10):1845-1849.
18	吕萍, 徐樨巍, 宋文琪, 等. 耐药肠球菌ermB基因与转座子Tnl545和Tn917的关系[J]. 中华微生物学和免疫学杂志, 2007, 27(8):730.
19	刘淑梅, 许淑珍. 390株肠球菌耐药性及Ⅰ类整合子的检测[J]. 中华医院感染学杂志, 2005(8):849-851.
20	吕萍, 徐樨巍, 宋文琪, 等. 儿童肠球菌多重耐药与Ⅰ类整合子的检测[J]. 检验医学, 2009, 24(3):219-222.

目的基因	引物序列(5′→3′)	长度(bp)
Ⅰ类整合子酶基因F	ACGAGCGCAAGGTTTCGGT	523
Ⅰ类整合子酶基因R	GAAAGGTCTGGTCATACATG
qacE△1-sull F	ATCGCAATAGTTGGCGAAGT	317
qacE△1-sull R	GCAAGGCGGAAACCCGCGCC
aac(6′)/aph(2′) F	CCAAGAGCAATAAGGGCATA	220
aac(6′)/aph(2′) R	CACTATCATAACCACTACCG
aph(3′) F	GCCGATGTGGATTGCGAAAA	292
aph(3′) R	GCTTGATCCCCAGTAAGTCA
ant(6)-Ⅰ F	ACTGGCTTAATCAATTTGGG	597
ant(6)-Ⅰ R	GCCTTTCCGCCACCTCACCG
tet M F	GTGTGACGAACTTTACCGAA	501
tet M R	GCTTTGTATCTCCAAGAACAC
erm B F	GAAAGGACTAACAAAA	533
erm B R	AGTAACGGTACTTAAATTGTTTAC
VanAF	GCTATTCAGCTGTACTC	738
VanAR	CAGCGGCCATCATACGG
VanBF	CATCGCCGTCCCCGAATTTCAAA	297
VanBR	GATGCGGAAGATACCGTGGCT

目的基因	引物序列(5′→3′)	长度(bp)
Ⅰ类整合子酶基因F	ACGAGCGCAAGGTTTCGGT	523
Ⅰ类整合子酶基因R	GAAAGGTCTGGTCATACATG
qacE△1-sull F	ATCGCAATAGTTGGCGAAGT	317
qacE△1-sull R	GCAAGGCGGAAACCCGCGCC
aac(6′)/aph(2′) F	CCAAGAGCAATAAGGGCATA	220
aac(6′)/aph(2′) R	CACTATCATAACCACTACCG
aph(3′) F	GCCGATGTGGATTGCGAAAA	292
aph(3′) R	GCTTGATCCCCAGTAAGTCA
ant(6)-Ⅰ F	ACTGGCTTAATCAATTTGGG	597
ant(6)-Ⅰ R	GCCTTTCCGCCACCTCACCG
tet M F	GTGTGACGAACTTTACCGAA	501
tet M R	GCTTTGTATCTCCAAGAACAC
erm B F	GAAAGGACTAACAAAA	533
erm B R	AGTAACGGTACTTAAATTGTTTAC
VanAF	GCTATTCAGCTGTACTC	738
VanAR	CAGCGGCCATCATACGG
VanBF	CATCGCCGTCCCCGAATTTCAAA	297
VanBR	GATGCGGAAGATACCGTGGCT

样本号	耐药表型	耐药基因组合
1	f，g	qacE△1-sull，aac(6′)/aph(2″)，tetM
2	d，f，g	qacE△1-sull，aph3′III，aac(6′)/aph(2″)，ant(6)I，tetM
3	d，e，f，g	qacE△1-sull，aph3′III，aac(6′)/aph(2″)，ant(6)I，tetM
4	e，f，g	aac(6′)/aph(2″)，tetM
5	c，d，e，g，h	qacE△1-sull，aac(6′)/aph(2″)
6	e，g	qacE△1-sull
7	b，e，f，g	qacE△1-sull，aph3′III，tetM

样本号	耐药表型	耐药基因组合
1	f，g	qacE△1-sull，aac(6′)/aph(2″)，tetM
2	d，f，g	qacE△1-sull，aph3′III，aac(6′)/aph(2″)，ant(6)I，tetM
3	d，e，f，g	qacE△1-sull，aph3′III，aac(6′)/aph(2″)，ant(6)I，tetM
4	e，f，g	aac(6′)/aph(2″)，tetM
5	c，d，e，g，h	qacE△1-sull，aac(6′)/aph(2″)
6	e，g	qacE△1-sull
7	b，e，f，g	qacE△1-sull，aph3′III，tetM

[1]	Ling Wei, Hui Li, Yi Chen. Current research status on colonization or infection and mother-to-child transmission of extended-spectrum β-lactamase-producing Enterobacteriaceae in pregnant and post-partum women [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(05): 517-521.
[2]	Jiajin Tu, Wuqiang Liao, Jinjing Liu, Zhipeng Tu, Yuangui Mao. Risk factors and prognosis analysis of Acinetobacter baumannii bloodstream infection in patients with severe burns [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(06): 491-497.
[3]	Wuqiang Liao, Jinjing Liu, Shiqiang Hu, Hongyan Zhang, Yuangui Mao, Jiajin Tu. Research progress on the prevalence of Acinetobacter baumannii in burn patients and its drug resistance mechanism [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2022, 17(06): 524-530.
[4]	Wei Li, Junqiao Mo. Genotype identification and drug resistance analysis of respiratory tract drug-resistant Haemophilus influenzae in children for the choice of antibiotic treatment [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2023, 17(05): 315-323.
[5]	Xiaoman Zhang, Xiaoqiu Ma, Zhengju Xu, Chunyu Zhang, Caiting He. Effect of drug-resistant mutations in reverse transcriptase region of hepatitis B virus on the level of serum hepatitis B surface antigen [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2023, 17(05): 324-332.
[6]	Haijin Zhang, Zengguo Wang, Huijun Cai, Bingtong Zhao. Distribution and drug resistance of pathogenic bacteria in neonates from 2020 to 2022 in Xi’an Children’s Hospital [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2023, 17(04): 222-229.
[7]	Changqing Cao, Xinyan Guo, Yuan Gao, Cun Zhang, Haili Tang, Dong Fan, Xiaojun Yang, Song Zhang, Huadong Zhao. Research progress on the involvement of tumor microenvironment in mediating trastuzumab resistance in HER2-positive breast cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(01): 90-95.
[8]	Xiaodan Wang, Yuan Wang, Xiangyu Cui, Xiaolei Ren. Analysis of pathogenic bacteria resistance of drug and high risk factors of death in urogenic sepsis after endoscopic surgery for upper urinary tract stones [J]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(06): 611-615.
[9]	Fayong Liu, Ping Hu, Li Dai. Analysis of distribution and drug resistance of bloodstream infection pathogens in with acquired pneumonia [J]. Chinese Journal of Lung Diseases(Electronic Edition), 2022, 15(05): 666-669.
[10]	Kaimei Gan, Jian Huang. Research progress in the effect of lung cancer stem cells on EGFR-TKI drug resistance [J]. Chinese Journal of Cell and Stem Cell(Electronic Edition), 2023, 13(01): 36-44.
[11]	Paerhati Nadina, Chen Huang. The role of cancer-associated fibroblasts in cancer progression and chemotherapy resistance of colorectal cancer [J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2023, 12(03): 241-247.
[12]	Jinqiu Li, Xiaofang Wei, Chengyu Wang. Analysis of bacteria spectrum change and drug sensitivity for peritoneal dialysis-associated peritonitis [J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2022, 11(05): 264-269.
[13]	Mengwei Hao, Zhen Chen, Tao Wei, Hui Zhang. Analysis of the relationship between serum miR-132-3p expression level and drug resistance in adult patients with epilepsy [J]. Chinese Journal of Brain Diseases and Rehabilitation(Electronic Edition), 2022, 12(06): 344-348.
[14]	Jingjing Li, Lei Zhai, Haiping Zhao, Bo Zheng. Polycystic kidney disease complicated with cystic infection caused by multi-drug resistant Klebsiella pneumoniae: a case report and literature review [J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(08): 920-923.
[15]	Fuqiang Diao, Xin Luo, Chunming Gu, Lingling Tang. Distribution and drug resistance of pathogenic bacteria in children with community-acquired Pneumonia in a hospital in Guangzhou [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(01): 38-44.

Please choose a citation manager

Content to export