Clinical evaluation of fluorescent staining for pathogen detection in vaginal discharge smear

doi:10.3877/cma.j.issn.2095-5820.2022.03.001

Abstract

Abstract:

Objective

To evaluate the clinical value of fluorescent staining in vaginal discharge smear for pathogen detection by comparing the results of direct microscopic examination and fluorescent method.

Methods

A total of 140 vaginal discharge samples were collected for the detection of clue cells, candida and Trichomonas vaginalis by using direct microscopic examination and fluorescent method, and the detection results were analyzed.

Results

For direct microscopic examination, the positive rates of clue cells, candida and Trichomonas vaginalis were 4.29%, 13.57% and 2.86%, respectively. For fluorescent method, the positive rates of clue cells, candida and Trichomonas vaginalis were 11.43%, 15.00% and 2.86%, respectively. The difference between direct microscopic examination and fluorescent method for the detection of clue cells was statistically significant (P＜0.05).

Conclusions

The positive rate of Trichomonas vaginalis and candida by fluorescent staining was consistent with that by direct microscopic examination, and the positive rate of clue cells was higher than that by wet staining. The fluorescent staining could be used for pathogen detection in vaginal discharge smear.

Key words: Vaginal discharge, Fluorescent dye, Candida, Clue cells, Trichomonas vaginalis

Xiaomao Yin, Peiming Wu, Yanting Li, Chuncai Hu, Zhiwei Wang. Clinical evaluation of fluorescent staining for pathogen detection in vaginal discharge smear[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2022, 10(03): 129-131,136.

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URL: https://zhlcsysgldzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-5820.2022.03.001

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References 30

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检测指标	阳性率（%）		χ²值	P值
检测指标	湿片法	荧光法	χ²值	P值
线索细胞	4.29	11.43	8.10	0.002
念珠菌	13.57	15.00	0.50	0.500
滴虫	2.86	2.86

检测指标	阳性率（%）		χ²值	P值
检测指标	湿片法	荧光法	χ²值	P值
线索细胞	4.29	11.43	8.10	0.002
念珠菌	13.57	15.00	0.50	0.500
滴虫	2.86	2.86

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