Effect of Coptis chinensis on ALS gene expression of Fluconazole resistant Candida albicans in biofilm state

doi:10.3877/cma.j.issn.2095-5820.2022.02.004

Abstract

Abstract:

Objective

To study the effect of different concentrations of Coptis chinensis extracts on the mRNA expression of ALS gene in Candida albicans under biofilm condition.

Methods

The drug-resistant strains of vaginal Candida albicans that formed biofilms were screened by the XTT reduction method. Under the intervention of Coptis chinensis at different concentrations, the expression of ALS3/ALS4 in the biofilm adhesion stage was detected by RT-PCR, and the inhibitory effect of Coptis chinensis on the expression of genes related to biofilm formation in Candida albicans was observed.

Results

When the concentration of Coptis chinensis was 125mg/L, the inhibition rate of candida albicans biofilm was 88.6% and 82.9%, respectively. The mRNA expression of adhesion related genes ALS3/ALS4 of Candida albicans in biofilm state was decreased under the intervention of Coptis chinensis with different concentrations. When the concentration of Coptis chinensis immersion solution was greater than 4.023mg/L, the mRNA expression relative level of ALS3/ALS4 under Coptis chinensis immersion intervention was significantly different from that of the negative control (P＜0.05).

Conclusion

Coptis chinensis inhibited the mRNA expression of Adhesion related gene ALS3 /ALS4 in Candida albicans in a dose-dependent manner.

Key words: Coptis chinensis, Candida albicans, Biofilm, Gene

Qianjun Li, Shuhong Huang, Yiyi Lei, Yonglin Feng. Effect of Coptis chinensis on ALS gene expression of Fluconazole resistant Candida albicans in biofilm state[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2022, 10(02): 89-94.

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References 29

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黄连浸液浓度（mg/L）	A_{492 nm}值
125.000	0.19±0.05^a
62.500	0.23±0.01^a
31.250	0.24±0.09^a
16.125	0.26±0.07^a
8.063	0.29±0.03^a
4.032	0.31±0.06^a
2.016	0.59±0.05^a
1.008	0.75±0.08^a
阴性对照组	0.84±0.10

黄连浸液浓度（mg/L）	A_{492 nm}值
125.000	0.19±0.05^a
62.500	0.23±0.01^a
31.250	0.24±0.09^a
16.125	0.26±0.07^a
8.063	0.29±0.03^a
4.032	0.31±0.06^a
2.016	0.59±0.05^a
1.008	0.75±0.08^a
阴性对照组	0.84±0.10

黄连浸液浓度（mg/L）	A_492nm值
黄连浸液浓度（mg/L）	24 h	48 h
125.000	0.175±0.003^a	0.191±0.005^a
62.500	0.221±0.002^a	0.234±0.006^a
31.250	0.237±0.004^a	0.241±0.009^a
16.125	0.246±0.007^a	0.253±0.004^a
8.063	0.274±0.008^a	0.287±0.003^a
4.032	0.294±0.006^a	0.306±0.005^a
2.016	0.342±0.005^a	0.479±0.008^a
1.008	0.468±0.008^a	0.504±0.006^a
阴性对照组	0.693±0.007	0.749±0.009

黄连浸液浓度（mg/L）	A_492nm值
黄连浸液浓度（mg/L）	24 h	48 h
125.000	0.175±0.003^a	0.191±0.005^a
62.500	0.221±0.002^a	0.234±0.006^a
31.250	0.237±0.004^a	0.241±0.009^a
16.125	0.246±0.007^a	0.253±0.004^a
8.063	0.274±0.008^a	0.287±0.003^a
4.032	0.294±0.006^a	0.306±0.005^a
2.016	0.342±0.005^a	0.479±0.008^a
1.008	0.468±0.008^a	0.504±0.006^a
阴性对照组	0.693±0.007	0.749±0.009

项目	黄连浸液浓度（mg/L）
项目	125.000	62.500	31.250	16.125	8.063	4.032	2.016	1.008	阴性对照组
24 h菌株数（株）	31	28	26	21	18	14	11	6	4
24 h抑制率（%）	88.6	80.0	74.2	60.0	51.4	40.0	31.4	17.1	11.4
48 h菌株数（株）	29	26	22	19	15	11	7	4	0
48 h抑制率（%）	82.9	74.2	62.9	54.3	42.9	31.4	20.0	11.4	0.0

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