Explore the effective alarm index of low mean volume of red blood cells affecting false increase of PLT

doi:10.3877/cma.j.issn.2095-5820.2022.02.005

Abstract

Abstract:

Objective

To analyze and compare the effect of low MCV specimens on the platelet count related indicators of the resistance method of Sysmex XN-2000 hematology analyzer, and search for the effective alarm indicators of PLT false increase caused by low MCV.

Methods

The PLT results of different MCV samples were collected by Sysmex XN-2000 hematology analyzer fluorescence method and resistance. The fluorescence method and resistance method difference of platelet detection (ΔPLT) under different grouping conditions were compared, and the correlation of cell parameters, cell histogram morphology and instrument alarm information with platelet difference (ΔPLT) was analyzed.

Results

ΔPLT in MCV＜80 fl group is higher than MCV≥80 fl group (P＜0.05); ΔPLT in MCV＜60 fl group is higher than MCV≥60 fl group (P＜0.05); when PLT-Ⅰ is between 150×10⁹/L and 600×10⁹/L, ΔPLT increases with the value increases (P＜0.05); PDW, MCHC, RDW-SD and ΔPLT have a certain correlation (P＜0.05), but R² is less than 0.95. The ratio of "Tail is not close to the abscissa" and "the tail is raised and forms the second peak" two kinds of platelet histogram phenomenon, red blood cell histogram "peak left shift" phenomenon, instrument alarm message "platelet abnormal distribution (PLT Abn Distribution)","Thrombocytosis","Anisocytosis","Fragments" and "Microcytosis" account was lower in ΔPLT≥100×10⁹/L group than ΔPLT＜100×10⁹/L group (P＜0.01).

Conclusions

Low MCV affected the PLT-Ⅰ results by the Sysmex XN-2000 hematologyanalyzer. The lower the MCV, the greater influence of PLT-I. When the PLT-Ⅰ≥70×10⁹/L, low MCV has greater influence on PLT-Ⅰ with the increase of PLT-Ⅰ. Combining the platelet histogram, the platelet instrument alarm message, the red blood cell histogram and the red blood cell instrument alarm message are helpful to judge the reliability of the resistance method of Sysmex XN-2000 hematologyanalyzer. It provides a comprehensive reference index and judgment basis for the timely discovery of low MCV affecting platelet count in clinical work.

Key words: Etythrocytes, Platelet count, Flow cytometry, Electric impedance

Meishuang Lin, Huidi Sun. Explore the effective alarm index of low mean volume of red blood cells affecting false increase of PLT[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2022, 10(02): 95-100.

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Figures/Tables 8

References 33

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组别	例数	PLT-I（×10⁹/L）	PLT-F（×10⁹/L）	ΔPLT（×10⁹/L）	t值	P值
MCV≥80 fl	138	86.530±78.918	77.530±75.817	9.000±17.331	-3.259	0.001
MCV＜80 fl	459	302.300±54.368	242.520±122.062	59.780±95.830	-3.259	0.001

组别	例数	PLT-I（×10⁹/L）	PLT-F（×10⁹/L）	ΔPLT（×10⁹/L）	t值	P值
MCV≥80 fl	138	86.530±78.918	77.530±75.817	9.000±17.331	-3.259	0.001
MCV＜80 fl	459	302.300±54.368	242.520±122.062	59.780±95.830	-3.259	0.001

MCV（fl）	例数	ΔPLT（×10⁹/L）	F值/P值
MCV＜60	63	112.900±168.209	14.759/0.000
60≤MCV＜70	261	59.380±76.427^a
70≤MCV＜80	135	35.760±71.096^ab

MCV（fl）	例数	ΔPLT（×10⁹/L）	F值/P值
MCV＜60	63	112.900±168.209	14.759/0.000
60≤MCV＜70	261	59.380±76.427^a
70≤MCV＜80	135	35.760±71.096^ab

PLT-Ⅰ（×10⁹/L）	例数	ΔPLT（×10⁹/L）
PLT-Ⅰ＜100	28	7.250±9.099^ab
100≤PLT-Ⅰ＜150	42	19.360±11.115^ac
150≤PLT-Ⅰ＜200	54	31.980±23.324^ab
200≤PLT-Ⅰ＜400	246	50.410±34.157
400≤PLT-Ⅰ＜600	67	87.040±60.856^d
PLT-Ⅰ≥600	22	273.450±337.571^d

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