Effect of inactivation of serum heated at 56 ℃ for 30 min on biochemical test results

doi:10.3877/cma.j.issn.2095-5820.2022.04.003

Abstract

Abstract:

Objective

According to the high infectivity and pathogenicity of SARS-CoV-2, this study investigated whether the serum heated at 56 ℃ for 30 minutes would affect the results of biochemical indicators, so as to provide an important reference for the laboratory to carry out the detection of routine biochemical indicators by heating inactivated specimens.

Methods

A total of 50 healthy people with physical examination in our hospital from January 2021 were enrolled in this study. The Beckman AU5800 automatic biochemical analyzer was used to determine the serum routine biochemical indicators before and after inactivation, and compared whether the inactivation method of heating at 56 ℃ for 30 min had any effect on the measured results.

Results

There were no significant differences in the results of serum total protein (TP), urea (UREA), creatinine (Cr), uric acid (UA), potassium (K), calcium (Ca), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) before and after inactivation (P＞0.05). The Bland Altman consistency analysis showed that the differences of serum total bilirubin (TBIL), sodium (Na), chlorine (Cl), phosphorus (P), carbon dioxide binding capacity (CO₂-CP), glucose (GLU), glycosylated serum protein (GSP), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), apolipoprotein a (Apo-A) and apolipoprotein B (Apo-B) before and after inactivation were clinically acceptable. However, serum direct bilirubin (DBIL), albumin (ALB), β₂-microglobulin (β₂-MG), homocysteine (HCY) did not have clinically acceptable consistency.

Conclusions

When laboratory process the specimen using the method of heating at 56°C for 30 minutes, it is necessary to take corrective measures for the biochemical indicators with significant influence, in order to provide accurate results.

Key words: SARS-CoV-2, Heat inactivation, Conventional biochemistry

Huan Li, Xiaoxiao Yin, Ruyuan Zong, Fumeng Yang, Qian Liu. Effect of inactivation of serum heated at 56 ℃ for 30 min on biochemical test results[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2022, 10(04): 204-209.

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Figures/Tables 3

项目	TEa（%）	来源	项目	TEa（%）	来源
TP	5	国家卫健委临床检验中心室间质评标准	Na	4	国家卫健委临床检验中心室间质评标准
UREA	8		Cl	4
Cr	12		P	10
UA	12		CO₂-CP	25
K	6		GLU	7
Ca	5		TC	9
TG	14		HDL-C	30
LDL-C	30		Apo-A	30
TBIL	15		Apo-B	30
DBIL	15		HCY	20
ALB	6		GSP	30	试剂说明书
β₂-MG	25

项目	灭活前	灭活后	t/Z值	P值
TP（g/L， $x ¯$ ±s）	76.15±3.85	76.15±3.79	0.041	0.9678
UREA（mmol/L， $x ¯$ ±s）	4.66±0.97	4.70±0.98	1.999	0.0512
Cr（µmol/L， $x ¯$ ±s）	63.94±11.35	63.88±11.21	0.219	0.8280
UA（µmol/L， $x ¯$ ±s）	309.40±72.33	309.70±72.29	0.853	0.3979
K（mmol/L， $x ¯$ ±s）	4.16±0.31	4.17±0.34	0.641	0.5243
Ca（mmol/L， $x ¯$ ±s）	2.28±0.07	2.28±0.07	0.805	0.4248
TG［mmol/L，M（Q₁，Q₃）］	1.06（0.82，1.43）	1.04（0.82，1.41）	-0.564	0.5730
LDL-C（mmol/L， $x ¯$ ±s）	2.71±0.53	2.70±0.53	1.948	0.0572
TBIL（µmol/L， $x ¯$ ±s）	15.56±6.54	15.72±6.63	5.936	＜0.0001
DBIL（µmol/L， $x ¯$ ±s）	2.49±0.88	2.39±0.83	3.974	0.0002
ALB（g/L， $x ¯$ ±s）	47.10±2.54	47.93±2.60	10.290	＜0.0001
β₂-MG［mg/L，M（Q₁，Q₃）］	1.50（1.40，1.70）	1.60（1.40，1.70）	-3.059	0.0022
Na（mmol/L， $x ¯$ ±s）	140.50±1.39	140.30±1.46	2.984	0.0044
Cl（mmol/L， $x ¯$ ±s）	102.30±1.85	102.00±1.80	5.719	＜0.0001
P（mmol/L， $x ¯$ ±s）	1.17±0.18	1.16±0.17	2.236	0.0226
CO₂-CP（mmol/L， $x ¯$ ±s）	22.23±2.33	21.07±2.17	14.900	＜0.0001
GLU［mmol/L，M（Q₁，Q₃）］	5.49（5.20，5.71）	5.53（5.17，5.80）	-2.596	0.0094
TC（mmol/L， $x ¯$ ±s）	4.36±0.68	4.41±0.68	7.562	＜0.0001
HDL-C（mmol/L， $x ¯$ ±s）	1.23±0.23	1.27±0.23	9.311	＜0.0001
Apo-A（g/L， $x ¯$ ±s）	1.63±0.22	1.66±0.22	6.657	＜0.0001
Apo-B（g/L， $x ¯$ ±s）	0.87±0.22	0.88±0.22	9.220	＜0.0001
HCY［µmol/L，M（Q₁，Q₃）］	7.30（5.50，9.70）	7.45（5.68，9.90）	-4.141	＜0.0001
GSP（mmol/L， $x ¯$ ±s）	2.25±0.23	2.18±0.23	5.377	＜0.0001

项目	灭活前	灭活后	t/Z值	P值
TP（g/L， $x ¯$ ±s）	76.15±3.85	76.15±3.79	0.041	0.9678
UREA（mmol/L， $x ¯$ ±s）	4.66±0.97	4.70±0.98	1.999	0.0512
Cr（µmol/L， $x ¯$ ±s）	63.94±11.35	63.88±11.21	0.219	0.8280
UA（µmol/L， $x ¯$ ±s）	309.40±72.33	309.70±72.29	0.853	0.3979
K（mmol/L， $x ¯$ ±s）	4.16±0.31	4.17±0.34	0.641	0.5243
Ca（mmol/L， $x ¯$ ±s）	2.28±0.07	2.28±0.07	0.805	0.4248
TG［mmol/L，M（Q₁，Q₃）］	1.06（0.82，1.43）	1.04（0.82，1.41）	-0.564	0.5730
LDL-C（mmol/L， $x ¯$ ±s）	2.71±0.53	2.70±0.53	1.948	0.0572
TBIL（µmol/L， $x ¯$ ±s）	15.56±6.54	15.72±6.63	5.936	＜0.0001
DBIL（µmol/L， $x ¯$ ±s）	2.49±0.88	2.39±0.83	3.974	0.0002
ALB（g/L， $x ¯$ ±s）	47.10±2.54	47.93±2.60	10.290	＜0.0001
β₂-MG［mg/L，M（Q₁，Q₃）］	1.50（1.40，1.70）	1.60（1.40，1.70）	-3.059	0.0022
Na（mmol/L， $x ¯$ ±s）	140.50±1.39	140.30±1.46	2.984	0.0044
Cl（mmol/L， $x ¯$ ±s）	102.30±1.85	102.00±1.80	5.719	＜0.0001
P（mmol/L， $x ¯$ ±s）	1.17±0.18	1.16±0.17	2.236	0.0226
CO₂-CP（mmol/L， $x ¯$ ±s）	22.23±2.33	21.07±2.17	14.900	＜0.0001
GLU［mmol/L，M（Q₁，Q₃）］	5.49（5.20，5.71）	5.53（5.17，5.80）	-2.596	0.0094
TC（mmol/L， $x ¯$ ±s）	4.36±0.68	4.41±0.68	7.562	＜0.0001
HDL-C（mmol/L， $x ¯$ ±s）	1.23±0.23	1.27±0.23	9.311	＜0.0001
Apo-A（g/L， $x ¯$ ±s）	1.63±0.22	1.66±0.22	6.657	＜0.0001
Apo-B（g/L， $x ¯$ ±s）	0.87±0.22	0.88±0.22	9.220	＜0.0001
HCY［µmol/L，M（Q₁，Q₃）］	7.30（5.50，9.70）	7.45（5.68，9.90）	-4.141	＜0.0001
GSP（mmol/L， $x ¯$ ±s）	2.25±0.23	2.18±0.23	5.377	＜0.0001

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