To investigate the impact of different proportions of sodium citrate anticoagulation and corrected blood collection volume on the results of thromboelastography (TEG) test in normal people and anemia patients respectively, so as to provide guidance for clinical blood collection.

29 patients with anemia admitted to The Third Affiliated Hospital of Guangzhou Medical University from July 2022 to March 2023 were selected as the observation group, and 20 normal individuals were selected for TEG test as normal group. The anemia patients were divided into two groups according to the correction formula, which were the pre-correction group and the post-correction group. The difference of TEG results before and after adjustment of blood collection volume was analyzed. The normal subjects were divided into four groups according to the ratio of anticoagulant to whole blood: group A (1:4), group B (1:9), group C (1:12) and group D (1:14). The TEG test was performed and the results of each group were statistically analyzed.

Reaction time (R) and coagulation time (K) in group A were significantly higher than those in group B (P<0.05). The angle and maximal amplitude (MA) in group A were lower than those in group B, and the differences were statistically significant (P<0.05). There was no significant difference between group C, D and group B (P<0.05); In patients with anemia (HCT>0.2), angle in the corrected group was higher than that in the pre-corrected group. In patients with anemia (HCT≤0.2), R and MA in the corrected group were higher than that in the pre-corrected group.

When the blood collection volume was insufficient, the results of TEG test were significantly different from the normal ratio (1:9), suggesting that the blood collection volume must be proper in clinical blood collection to ensure the accuracy of experimental results. There was a significant difference in TEG test before and after correction in anemia patients. In order to improve the accuracy of the test, the blood collection test should be conducted after correcting the amount of anticoagulant or blood collection.