Protective effect of HO-1 overexpression against hypoxia-reoxygenation injury of human astrocytes of spinal cord

doi:10.3877/cma.j.issn.2095-5820.2018.03.007

Abstract

Abstract:

Objective

To investigate the protective effect of overexpressed heme oxygenase-1(HO-1) in hypoxia-reoxygenation of human astrocytes of spinal cord(HA-sp).

Methods

Hypoxia-reoxygenation mode was established by oxygen provision after CoCl₂ stimulation. The expression of HO-1 under different stimulation was detected by Western blotting. MTT assay was used to examine the relative survival of cell under hypoxia-reoxygenation as well as after up-regulated HO-1.

Results

HO-1 was upregulated by hypoxia/re-oxygen stimulation. The relative survival rate of HA-sp under hypoxia-reoxygenation was significantly decreased after HO-1 protein overexpression was inhibited by ZnPPIX (P<0.01), while the relative survival rate of HA-sp which was pretreated by CoPP was significantly increased compared with that of cells under hypoxia-reoxygenation (P<0.01).

Conclusion

HO-1 overexpression by CoPP exerts protective effect on hypoxia-reoxygenation injury of HA-sp. The result of this study may offer new thought for the therapy of hypoxia-reoxygenation injury of human spinal cord in the future.

Key words: Heme oxygenase-1, Human astrocytes of spinal cord, Hypoxia-reoxygenation, Survival rate

Fei Liang, Wei Wei, Zerong Yao, Xiaoze Li. Protective effect of HO-1 overexpression against hypoxia-reoxygenation injury of human astrocytes of spinal cord[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2018, 06(04): 222-225.

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References 13

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