Application of two kinds of blood culture flask in the analysis of blood stream infection of AIDS patients in a hospital in Kunming in recent 5 years

doi:10.3877/cma.j.issn.2095-5820.2019.04.005

Abstract

Abstract:

Objective

To investigate the pathogen distribution of blood stream infection among AIDS patients in Kunming hospital in recent 5 years. The detection of different pathogens in two blood culture flasks was compared.

Methods

The results of two blood culture vials (ordinary blood culture and mycobacterium/fungal blood culture) used in hospitalized AIDS patients from 2013 to 2017 were retrospectively analyzed.

Results

The positive rate of ordinary blood culture was 5.8%; the top 5 pathogens were Talaromyces marneffei, Cryptococcus neoformans, coagulase negative Staphylococcus, Staphylococcus aureus and Escherichia coli. The positive rate of Mycobacterium/fungus blood culture was 12.8%, and the top 5 pathogens were Mycobacterium, marneffei, Cryptococcus neoformans, Escherichia coli and coagulase negative Staphylococcus.

Conclusions

From 2013 to 2017, the main pathogens of blood stream infection in AIDS patients were fungi, and and the main fungi were Talaromyces marneffei and Cryptococcus neoformans. The detection rate of Mycobacterium was 4.8% using Mycobacterium/fungus blood culture bottle. Mycobacterium was the second only pathogen causing blood stream infection in AIDS patients, and it could not be ignored. Ordinary blood culture flask combined with Mycobacterium/fungus blood culture flask can increase the detection of blood stream infection pathogens in AIDS patients.

Key words: AIDS, Blood stream infection, Pathogenic, Fungi, Mycobacterium, Blood culture

Junhua Su, Jianrui Zhang, Xiaofei Li, Shaoli Qian, Yi Xiao, Hongwei Liu. Application of two kinds of blood culture flask in the analysis of blood stream infection of AIDS patients in a hospital in Kunming in recent 5 years[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2019, 07(04): 210-213.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhlcsysgldzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-5820.2019.04.005

https://zhlcsysgldzzz.cma-cmc.com.cn/EN/Y2019/V07/I04/210

Figures/Tables 3

References 13

1	周庭银，倪语星，王明贵. 血流感染实验诊断与临床诊治[M]. 上海：上海科学技术出版社, 2011:15-16.
2	唐秀文，张晶. 2350例艾滋病患者血液培养病原体和药敏结果分析[J]. 广西医学, 2010,32(7):782-785.
3	徐国红，李青峰，刘应芬，等. 成都市艾滋病患者血流感染病原菌分布及耐药性分析[J].中华传染病杂志, 2016,34(10):609-612.
4	何红，徐丹，沈辉，等. 2010-2014年长沙市某医院AIDS住院患者感染菌群分布[J]. 实用预防医学, 2017,24(2):202-204.
5	谢宁. 3905例艾滋病患者血培养病原体分布结果分析[J]. 海南医学, 2012,23(9):89-91.
6	李敏，马小亮，徐新民，等. 艾滋病血流感染的研究综述[J]. 中医临床研究, 2015,7(34): 145-146.
7	中华医学会感染病学分会艾滋病学组. 艾滋病诊疗指南(2011版)[J]. 中华临床感染病杂志, 2011,4(6):321-330.
8	朱明利，黄谷良，林特夫. 分支杆菌菌血症研究进展[J]. 中华结核和呼吸杂志, 2001,24(10):45-47.
9	张峣，于兰，唐志荣，等. 血液结核分枝杆菌液体培养在HIV感染者结核筛查中的应用评价[J/CD]. 中华实验和临床感染病杂志：电子版, 2011,5(4):430-436.
10	曾昭英，赵秀英，于艳华，等. 血培养联合PCR对HIV感染者和AIDS患者分枝杆菌血症诊断的分析[J]. 检验医学, 2011,26(4):235-238.
11	Crump J, Ramadhani HO, Morrissey AB, et al. Invasivebacterial and fungal infections among hospitalized HIV-infected and HIV-uninfected adults and adolescenti in northen Tanzani[J]. Clin Infect Dis, 2011,52(3):341-348.
12	Taniguchi T, Ogawa Y, Kasai DA, et al. Three cases of fungemia in HIV-Infected patients diagnosed through the use of mycobacterial blood culture bottles[J]. Internal Medicine, 2010, 49(19):2179-2183.
13	李光辉，朱德妹，汪复，等. 2011年中国CHINET血培养临床分离菌的分布及耐药性[J]. 中国感染与化疗杂志, 2013,13(4):241-247.

年份	普通血培养			分枝杆菌/真菌血培养
年份	培养瓶数	阳性数	阳性率(%)	培养瓶数	阳性数	阳性率(%)
2013	1152	68	5.9	576	71	12.3
2014	1174	86	7.3	587	81	13.6
2015	1274	86	6.8	637	80	12.6
2016	1252	55	4.4	626	76	11.9
2017	1112	56	5.0	556	75	13.5
合计	5964	351	5.9	2982	383	12.8

年份	普通血培养			分枝杆菌/真菌血培养
年份	培养瓶数	阳性数	阳性率(%)	培养瓶数	阳性数	阳性率(%)
2013	1152	68	5.9	576	71	12.3
2014	1174	86	7.3	587	81	13.6
2015	1274	86	6.8	637	80	12.6
2016	1252	55	4.4	626	76	11.9
2017	1112	56	5.0	556	75	13.5
合计	5964	351	5.9	2982	383	12.8

培养瓶	马尔尼菲篮状菌(株)	新型隐球菌(株)
普通血培养瓶	89	76
分枝杆菌/真菌瓶	128	57
两种瓶均检出	66	45
仅普通血培养瓶检出	23	21
仅分枝杆菌/真菌瓶检出	62	12

培养瓶	马尔尼菲篮状菌(株)	新型隐球菌(株)
普通血培养瓶	89	76
分枝杆菌/真菌瓶	128	57
两种瓶均检出	66	45
仅普通血培养瓶检出	23	21
仅分枝杆菌/真菌瓶检出	62	12

[1]	Zhijia Wen, Haobo Zhong, Shaowei Zheng, Xu Li, Yin Wang, Guowei Zeng, Shengjie Wang, Chunhan Sun. Research progress of periprosthesis fungal infection after artificial joint replacement [J]. Chinese Journal of Joint Surgery(Electronic Edition), 2023, 17(01): 60-64.
[2]	Yukun Guo, Yu′an Zhu, Xiaoming Fan, Feng Zhu, Bing Ma. Research progress on pathogenic mechanism of Vibrio vulnificus [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2022, 17(01): 81-84.
[3]	Jie Tan, Senlin Zhan, Guofang Deng, Peize Zhang. A case of Mycobacterium Columbia disseminated infection caused by anti-interferon γ autoantibody syndrome [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2022, 16(03): 210-214.
[4]	Qiangsheng Feng, Yuejuan Song, Xiaoqin Ha, Baodong Gao. Clinical characteristics, pathogen distribution and infection biomarkers of patients with invasive Candida infection [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition), 2022, 16(01): 47-53.
[5]	Xiaoyin Zhang, Yang Yu. Research progress on the occurrence and development of chronic kidney disease by Porphyromonas gingivalis [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2023, 17(05): 328-334.
[6]	Yuanyuan Xiang, Weina Zhang, Yulei Huang, Lin Wang, Jing Yang, Runfu Wang, Dongsheng Yu, Xiaohuan Zhang. Analysis of pathogenic characteristics and related factors of nosocomial infection in inpatients of oral and maxillofacial surgery [J]. Chinese Journal of Stomatological Research(Electronic Edition), 2021, 15(03): 142-147.
[7]	Xiaodan Wang, Yuan Wang, Xiangyu Cui, Xiaolei Ren. Analysis of pathogenic bacteria resistance of drug and high risk factors of death in urogenic sepsis after endoscopic surgery for upper urinary tract stones [J]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(06): 611-615.
[8]	Wei Wang, Xiangzhu Du, Yue Ma, Xiaofang Qi. Study of the pathogenic bacteria and risk factors for patients with modified indwelling catheter-associated urinary tract infection [J]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(01): 78-81.
[9]	Xiaopeng Zeng, Lixin Fan, Chengsong Li, Kunpeng Liu, Xiongcai Zhou, Zhihua Wang, Xiaosheng Li. Relationship between CD₄⁺ T-lymphocyte levels and perioperative infection in urological endoscopic surgery in HIV/AIDS patients [J]. Chinese Journal of Endourology(Electronic Edition), 2021, 15(06): 502-506.
[10]	Fayong Liu, Ping Hu, Li Dai. Analysis of distribution and drug resistance of bloodstream infection pathogens in with acquired pneumonia [J]. Chinese Journal of Lung Diseases(Electronic Edition), 2022, 15(05): 666-669.
[11]	Hongxia Zhang, Binglei Ge, Caihong Zuo, Jun Li, Qing Zhang, Caiyi Zhang, Zhaoguo Huang, Jian Chen. Analysis of pathogenic bacteria in orthopedic surgical site infection of class I incision [J]. Chinese Journal of Shoulder and Elbow(Electronic Edition), 2021, 09(03): 263-267.
[12]	Jinqiu Li, Xiaofang Wei, Chengyu Wang. Analysis of bacteria spectrum change and drug sensitivity for peritoneal dialysis-associated peritonitis [J]. Chinese Journal of Kidney Disease Investigation(Electronic Edition), 2022, 11(05): 264-269.
[13]	Xiaosu Wang, Zheng Dai, Jiajia Zhu, Qichao Li, Litao Zhang. Comparative study on the detection ability of BacT/ALERT two blood culture systems and eight blood culture bottles for simulated bacteremia specimens [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(04): 207-213.
[14]	Xiaorong Hu, Xiaolong Li, Juan Ouyang, Siyu He, Jiangqin Song. Clinical evaluation of TTD and antibiotic adsorption capacity of different adsorption medium culture flasks [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(02): 84-89,94.
[15]	Junqing Tan, Xiaojun Li, Yuwei He, Aiwen Li. Strategies for using PDCA cycle to improve the biosafety management of pathogenic microorganism laboratories in hospitals [J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2022, 10(04): 193-198.

Please choose a citation manager

Content to export