Home    中文  
 
  • Search
  • lucene Search
  • Citation
  • Fig/Tab
  • Adv Search
Just Accepted  |  Current Issue  |  Archive  |  Featured Articles  |  Most Read  |  Most Download  |  Most Cited

Chinese Journal of Clinical Laboratory Management(Electronic Edition) ›› 2021, Vol. 09 ›› Issue (04): 217-221. doi: 10.3877/cma.j.issn.2095-5820.2021.04.005

• Experiment Researchs • Previous Articles     Next Articles

Relationship between KRAS, NRAS, BRAF and PIK3CA gene mutations and clinicopathological features in colorectal cancer

Zhijian Lin1, Yunjian Xu1, Guangming Wen2,()   

  1. 1. Department of Medical Laboratory, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Guangdong 510120, China
    2. Department of Gastrointestinal Surgery, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Guangdong 510120, China
  • Received:2021-01-19 Online:2021-11-28 Published:2022-01-04
  • Contact: Guangming Wen

Abstract:

Objective

To study the relationship between KRAS, NRAS, BRAF and PIK3CA mutations and the clinicopathological features in patients with colorectal cancer (CRC).

Methods

The 189 samples of colorectal cancer were collected by surgical excision or biopsy,and DNA were extracted. The mutations of KRAS, NRAS, BRAF and PIK3CA genes were detected by amplification refractory mutation system, and its relationship with clinicopathological features of CRC was analyzed.

Results

The mutations rate of KRAS were 49.7%, in most exon2 mutations; the mutations rate of NRAS were 3.7%; the mutations rate of BRAF were 4.2%; the mutations rate of PIK3CA were 2.6%. There were 5 patients with PIK3CA gene mutations, all of them had KRAS mutations. KRAS mutations were significantly more prevalent in tumors in the proximal colon than in tumors in the distal colon (P=0.048). NRAS mutations rate in patients without nerve bundle invasion were remarkably higher than in patients with nerve bundle invasion (P=0.001). BRAF mutations were significantly more prevalent in tumors in T4 than in tumors in T1-T3 (P=0.029). BRAF mutations were also more common in poorly differentiated tumors than in well and moderately differentiated tumors (P=0.023).

Conclusions

The mutations rate of KRAS gene is high, KRAS, NRAS and BRAF mutations were significantly correlated with the clinicopathological features of CRC.

Key words: Colorectal cancer, Gene, Mutation

京ICP 备07035254号-20
Copyright © Chinese Journal of Clinical Laboratory Management(Electronic Edition), All Rights Reserved.
Tel: 020-81341720 Fax: 020-37103505 E-mail: clinlab@cma.org.cn
Powered by Beijing Magtech Co. Ltd