MCC950 inhibites rotenone-induced activation of neuroinflammation and cell apoptosis

doi:10.3877/cma.j.issn.2095-5820.2025.02.002

Abstract

Abstract:

Objective

To investigate the toxic effects of rotenone on dopaminergic neurons and the protective effects and mechanisms of MCC950 against rotenone-induced neuron damage.

Methods

The rotenone-induced SN4741 Parkinson's disease neuron cell model was constructed. The effects of rotenone on the viability of SN4741 cells were detected by cell counting kit-8 method and lactate dehydrogenase release test.Propidium iodide staining was used to detect the effect of rotenone on apoptosis of SN4741 cells. The effects of rotenone on the expression of NOD-like receptor pyrin domain containing 3 (NLRP3) and its downstream inflammatory factors in SN4741 cells were detected by Western blot and ELISA. The effect of NLRP3 inhibitor MCC950 on rotenone-induced SN4741 cell inflammation and cell apoptosis was investigated.

Results

With the increase of rotenone concentration, SN4741 cell viability decreased and apoptosis increased (P＜0.01).The expressions of NLRP3, IL-1β and IL-18 in rotenone treatment group were increased compared with those in control group (P＜0.01). Rotenone plus MCC950 treatment group alleviated the decreased cell viability, increased apoptosis and increased expression of NLRP3, IL-1β and IL-18 caused by rotenone treatment group.

Conclusion

Rotenone can induce neuronal apoptosis and cell death through activation of neuroinflammation, and MCC950 attenuates rotenone-induced neuroinflammation and cell apoptosis.

Key words: Parkinson's disease, rotenone, dopamine neurons, neuroinflammation

Qiang Ming, Rui Liu, Chunlai Fu, Liya Gong, Yingying Hu, Jialong Chen, He Zhang. MCC950 inhibites rotenone-induced activation of neuroinflammation and cell apoptosis[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2025, 13(02): 72-78.

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Figures/Tables 4

References 16

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