Rotenone promotes ferroptosis of dopaminergic neurons by reducing the expression of MCU protein

doi:10.3877/cma.j.issn.2095-5820.2023.02.002

Abstract

Abstract:

Objective

To study the mechanism of mitochondrial calcium uniporter (MCU) in regulating rotenone induced ferroptosis in dopaminergic neurons.

Methods

Immunoblotting test and lipid oxidation kit were used to detect the expression of MCU protein and the level of ferroptosis before and after rotenone treatment. The changes of the expression of MCU protein and the level of ferroptosis after rotenone treatment were detected.

Results

After rotenone treatment, the expression of glutathione peroxidase - 4 and ferritin heavy chain polypeptide - 1 protein in dopaminergic neurons were decreased, and the level of intracellular oxidative stress was increased; the expression of MCU protein and the level of ferroptosis were decreased after the recovery of MCU protein expression.

Conclusions

Rotenone can promote ferroptosis of dopaminergic neurons by reducing the expression of MCU protein.

Key words: MCU, Parkinson's disease, rotenone, ferroptosis

Ailun Xie, Dongyan Zheng, Ziwei Cai, Renjian Lu, Yongming Peng, He Zhang, Jialong Chen. Rotenone promotes ferroptosis of dopaminergic neurons by reducing the expression of MCU protein[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(02): 71-78.

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References 36

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试剂	体积/ml
高糖DMEM培养基	500.0
30%葡萄糖	8.2
L-谷氨酰胺	3.8
青霉素-链霉素抗生素	5.4
胎牛血清	54.0

试剂	体积/ml
高糖DMEM培养基	500.0
30%葡萄糖	8.2
L-谷氨酰胺	3.8
青霉素-链霉素抗生素	5.4
胎牛血清	54.0

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