With the rapid development of modern emergency medicine and laboratory medicine, emergency medical laboratory has acquired much more attention. The industry has also issued various relevant guidelines and consensus, but most of guidelines do not have clear requirements and measures as various standards are scattered, which makes emergency medical laboratories exist many problems and hidden dangers that affect laboratory safety and quality. The author combined the recently launched "Establishment of emergency medical laboratory ability and standardization of Chinese experts′ consensus", various related industry norms, and the multi-year practice of emergency medical laboratory to conduct a comprehensive review of the establishment and operation of emergency medical laboratory for exchange and reference.