切换至 "中华医学电子期刊资源库"

中华临床实验室管理电子杂志 ›› 2014, Vol. 02 ›› Issue (03) : 148 -151. doi: 10.3877/cma.j.issn.2095-5820.03.004

综述

埃博拉病毒的实验室检测
马硝惟1, 李敏1,()   
  1. 1.200127,上海交通大学医学院附属仁济医院检验科
  • 收稿日期:2014-08-01 出版日期:2014-08-28
  • 通信作者: 李敏
  • 基金资助:
    国家自然基金资助项目(81322025、81171623、81371875)上海市卫生系统优秀青年人才计划(XYQ2011039)上海市曙光人才计划项目(12SG03)

Testing methods in clinical laboratory for Ebola virus

Xiaowei MA1, Min LI1,()   

  1. 1.Department of Clinical Laboratory, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
  • Received:2014-08-01 Published:2014-08-28
  • Corresponding author: Min LI
引用本文:

马硝惟, 李敏. 埃博拉病毒的实验室检测[J/OL]. 中华临床实验室管理电子杂志, 2014, 02(03): 148-151.

Xiaowei MA, Min LI. Testing methods in clinical laboratory for Ebola virus[J/OL]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2014, 02(03): 148-151.

2014年埃博拉病毒感染在西非引起高致死性出血热疫情流行,目前对该病毒缺乏特异性的疫苗和治疗方案,早期快速诊断能明显降低感染死亡率。因此,埃博拉病毒早期快速检测方法的建立无论在疫情国家还是非疫情国家都非常重要。本文总结了埃博拉病毒的现有检测技术及进展。

Outbreaks of infection by Ebola virus (EBOV) occurred in West Africa caused the hemorrhagic diseases with high lethality in 2014. Till now, there is not any specific vaccine or treatment for this disease; early and rapid diagnosis of EBOV infection can significantly reduce the mortality. Therefore, establishment of rapid methods for detecting EBOV infection in clinical laboratories is very important in those countries with or without epidemic situation. Here we reviewed the recent progress of testing methods in clinical laboratory for Ebola virus infection.

1
World Health Organization. Ebola haemorrhagic fever [DB/OL].[2012-09] http://www.who.int/mediacentre/factsheets/fs103/en/
2
Peters CJ, LeDuc JW. An introduction to Ebola: the virus and the disease [J]. J Infect Dis, 1999, 179 (Suppl): ix-xvi.
3
Leroy EM, Kumulungui B, Pourrut X, et al. Fruit bats as reservoirs of Ebola virus [J]. Nature, 2005, 438 (7068): 575-576.
4
Feldmann H, Jones S, Klenk HD, et al. Ebola virus: from discovery to vaccine [J]. Nat Rev Immunol, 2003, 3 (8): 677-685.
5
Geisbert TW, Jahrling PB. Exotic emerging viral diseases: progress and challenges [J]. Nat Med, 2004, 10(12 Suppl): 110-121.
6
Gatherer D. The 2014 Ebola virus disease outbreak in west Africa [J].J Gen Virol, 2014, 95(Pt 8): 1619-1624.
7
Bente D, Gren J, Strong JE, et al. Disease modeling for Ebola and Marburg viruses [J]. Dis Model Mech, 2009, 2(1-2): 12-17.
8
Snowden FM. Emerging and reemerging diseases: a historical perspective [J]. Immunol Rev, 2008, 225: 9-26.
9
Tambo E, Ugwu EC, Ngogang JY. Need of surveillance response systems to combat Ebola outbreaks and other emerging infectious diseases in African countries [J]. Infect Dis Poverty, 2014, 3: 29.
10
刘阳, 史子学, 马玉堃, 等. SYBR GreenⅠ荧光定量RT-PCR检测埃博拉病毒方法的建立 [J]. 病毒学报, 2012, 28(5): 567-571.
11
Huang Y, Wei H, Wang Y, et al. Rapid detection of filoviruses by realtime TaqMan polymerase chain reaction assays [J]. Virol Sin, 2012,27(5): 273-277.
12
Towner JS, Rollin PE, Bausch DG, et al. Rapid diagnosis of Ebola hemorrhagic fever by reverse transcription-PCR in an outbreak setting and assessment of patient viral load as a predictor of outcome [J]. J Virol 2004, 78(8): 4330-4341.
13
Okware SI, Omaswa FG, Zaramba S, et al. An outbreak of Ebola in Uganda [J]. Trop Med Int Health, 2002, 7(12): 1068-1075.
14
Leroy EM, Baize S, Lu CY, et al. Diagnosis of Ebola haemorrhagic fever by RT-PCR in an epidemic setting [J]. J Med Virol, 2000, 60(4):463-467.
15
夏文英, 郑夔, 黄吉城, 等. 埃博拉病毒检测基因芯片的制备初探[J]. 中国国境卫生检疫杂志, 2011, 34(3): 150-154.
16
Gire SK, Goba A, Andersen KG, et al. Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak [J/OL].Science, 2014; http://www.sciencemag.org/content/early/2014/08/27/science.1259657?cited-by=yes&legid=sci;science.1259657v1#cited-by.
17
Lucht A, Grunow R, Otterbein C, et al. Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus [J]. Med Microbiol Immunol, 2004, 193(4): 181-187.
18
Lucht A, Grunow R, Möller P, et al. Development, characterization and use of monoclonal VP40-antibodies for the detection of Ebola virus [J].J Virol Methods, 2003, 111(1): 21-28.
19
Lucht A, Formenty P, Feldmann H, et al. Development of an immunofiltration-based antigen-detection assay for rapid diagnosis of Ebola virus infection [J]. J Infect Dis, 2007, 196(Suppl 2): S184-S192.
20
Rowe AK, Bertolli J, Khan AS, et al. Clinical, virologic, and immunologic follow-up of convalescent Ebola hemorrhagic fever patients and their household contacts, Kikwit, Democratic Republic of the Congo [J]. J Infect Dis, 1999, 179(Suppl): S28-S35.
21
Baize S, Leroy EM, Georges-Courbot MC, et al. Defective humoral responses and extensive intravascular apoptosis are associated with fatal outcome in Ebola virus-infected patients [J]. Nat Med, 1999,5(4): 423-426.
22
Busico KM, Marshall KL, Ksiazek TG, et al. Prevalence of IgG antibodies to Ebola virus in individuals during an Ebola outbreak,Democratic Republic of the Congo, 1995 [J]. J Infect Dis, 1999,179(Suppl): S102-S107.
23
Ksiazek TG, West CP, Rollin PE, et al. ELISA for the detection of antibodies to Ebola viruses [J]. J Infect Dis, 1999, 179(Suppl):S192-S198.
24
Saijo M, Niikura M, Ikegami T, et al. Laboratory diagnostic systems for Ebola and Marburg hemorrhagic fevers developed with recombinant proteins [J]. Clin Vaccine Immunol, 2006, 13(4): 444-451.
25
=Pattyn S, van der Groen G, Courteille G, et al. Isolation of Marburglike virus from a case of haemorrhagic fever in Zaire [J]. Lancet, 1977,1(8011): 573-574.
26
Le Guenno B, Formenty P, Formentry P, et al. Isolation and partial characterisation of a new strain of Ebola virus [J]. Lancet, 2006,367(9513): 816.
27
Bowen ETW, Lloyd G, Harris WJ, et al. Viral hæmorrhagic fever in Southern Sudan and Northern Zaire[J]. Lancet, 1977, 1(8011): 571-573.
28
Zaki SR, Shieh WJ, Greer PW, et al. A novel immunohistochemical assay for the detection of Ebola virus in skin: implications for diagnosis, spread, and surveillance of Ebola hemorrhagic fever.Commission de Lutte contre les Epidémies à Kikwit [J]. J Infect Dis,1999, 179(Suppl): S36-S47.
29
Ikegami T1, Miranda ME, Calaor AB,et al. Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996[J]. Exp Anim. 2002 Oct, 51(5): 447-455.
30
Geisbert TW, Jahrling PB. Differentiation of filoviruses by electron microscopy [J]. Virus Res, 1995, 39 (2-3): 129-150.
31
Geisbert TW, Rhoderick JB, Jahrling PB. Rapid identification of Ebola virus and related filoviruses in fluid specimens using indirect immunoelectron microscopy [J]. J Clin Pathol, 1991, 44(6): 521-522.
32
Bharat TAM, Noda T, Riches JD, et al. Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography[J]. Proc Natl Acad Sci U S A, 2012, 109(11): 4275-4280.
[1] 高建松, 陈晓晓, 冯婷, 包剑锋, 魏淑芳, 潘林. 基于超声瞬时弹性成像的多参数决策树模型评估慢性乙型肝炎患者肝纤维化等级[J/OL]. 中华医学超声杂志(电子版), 2023, 20(09): 923-929.
[2] 钱警语, 郑明明. 《2024意大利妇产科学会非侵入性和侵入性产前诊断指南》解读[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(05): 486-492.
[3] 刘轩瑶, 赵晓曦. 孕妇常见产前筛查指标单独及联合检测对胎儿染色体异常的预测价值[J/OL]. 中华妇幼临床医学杂志(电子版), 2023, 19(06): 657-664.
[4] 李亚萍, 张萌, 李博驹, 刘晨瑞, 苟国娥, 李嘉昕, 张玉凤, 席淼, 邓慧玲. 干扰素诱导蛋白16-干扰素基因刺激因子通路在柯萨奇病毒A6型感染手足口病患儿的表达及其临床意义[J/OL]. 中华实验和临床感染病杂志(电子版), 2024, 18(03): 135-141.
[5] 胡宁宁, 赵延荣, 王栋, 王胜亮, 郭源. FMNL3与肝细胞癌肝移植受者预后的相关性研究[J/OL]. 中华移植杂志(电子版), 2024, 18(05): 283-288.
[6] 司钦亮, 毕世龙, 焦慧骁, 李世照, 陈哲禹, 武玉东. 精索去分化脂肪肉瘤两例并文献复习[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 585-590.
[7] 刘中文, 刘畅, 高洋, 刘东, 林世庆, 杨建华, 赵福义. 尿液microRNA-326与腹腔镜根治性膀胱切除术治疗膀胱癌患者预后的相关性研究[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(04): 386-391.
[8] 杨慧, 郭丽娟, 冯晓丹, 李静, 黄成谋, 蔡兴锐, 覃英娇, 王远礼. 非小细胞肺癌铂类药物耐药mi RNA表达特征及预测分析[J/OL]. 中华肺部疾病杂志(电子版), 2024, 17(05): 719-724.
[9] 张桂萍, 丘勇林, 湛绮婷, 孙乐栋. 晚期非小细胞肺癌血清Ape1/Ref-1对放射性肺损伤发生的预测意义[J/OL]. 中华肺部疾病杂志(电子版), 2024, 17(04): 519-523.
[10] 刘涵, 沈强, 张蓝月, 陈健. 糖尿病视网膜病变分子生物标志物的研究进展[J/OL]. 中华眼科医学杂志(电子版), 2023, 13(06): 376-380.
[11] 黄莉吉, 王婷, 朱鹏飞, 刘敬顺, 余江毅, 谢绍锋. 芪葵颗粒联合火把花根片对G3A3期糖尿病肾病的疗效及对血清miRNA-21的影响[J/OL]. 中华临床医师杂志(电子版), 2023, 17(12): 1247-1252.
[12] 辛彩焕, 熊辉. 非疫区36例布鲁菌病患者的临床特征及诊疗分析[J/OL]. 中华临床医师杂志(电子版), 2023, 17(09): 927-931.
[13] 陶莹, 李晓光, 朱桃红, 杨彦, 陈琦. Westgard西格玛规则在中孕期母血清学产前筛查质量管理中的应用[J/OL]. 中华临床实验室管理电子杂志, 2024, 12(02): 65-69.
[14] 康嘉乐, 李嘉华, 李志方, 陈荣彬, 何梦华, 魏洁. 不同核酸提取方案对EB病毒核酸质量及扩增影响分析[J/OL]. 中华临床实验室管理电子杂志, 2024, 12(02): 80-85.
[15] 郭彬焰, 褚衍六. 胃癌分期评估模型的研究现状与展望[J/OL]. 中华胃肠内镜电子杂志, 2024, 11(02): 120-122.
阅读次数
全文


摘要


AI


AI小编
你好!我是《中华医学电子期刊资源库》AI小编,有什么可以帮您的吗?