探讨Th1/Th2/Th17型细胞因子在非小细胞肺癌(non-small cell lung cancer，NSCLC)治疗过程中的临床意义。

收集56例初步诊断为NSCLC患者化疗前、第1~4次化疗的外周血标本，用流式细胞术微球阵列法(flow cytometry microsphere array，CBA)检测其血清中Th1/Th2/Th17型细胞因子(IL-2、IL-4、IL-6、IL-10、IL-17、TNF-α、IFN-γ)水平，并分析上述指标与NSCLC患者各个治疗阶段的关系。

在第4次化疗后，IL-2、IL-4、IL-10、TNF-α水平显著上升，与前3次差异有统计学意义(P<0.05)。IL-6、IL-17水平均有显著下降，并与化疗前差异有统计学意义(P<0.05)。IFN-γ水平在化疗阶段存在波动，其第2~4次化疗均相较于第1次化疗存在显著性差异(P<0.05)。

Th1/Th2/Th17型细胞因子水平随着NSCLC化疗病情的缓和达到趋势性稳定，说明研究细胞因子失衡导致的免疫紊乱在NSCLC诊疗中有重要意义。

To investigate the clinical significance of Th1/Th2/Th17 cytokines during the treatment of non-small cell lung cancer (NSCLC).

Fifty-six cases preliminarily diagnosed as non-small cell lung cancer were enrolled in this study. The peripheral blood samples were collected before patients receiving, the first, the second, the third and the fourth cycle of chemotherapy. The serum cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, TNF-α and IFN-γ) were detected by flow cytometry microsphere array (CBA) method. The relationship between these indices and the treatment stages of patients with non-small cell lung cancer was analyzed.

After the fourth cycle of chemotherapy, the levels of IL-2, IL-4, IL-10 and TNF-α increased significantly, and there was a significant difference between the three groups (P<0.05). The levels of IL-6 and IL-17 were significantly decreased compared with those before chemotherapy (P<0.05). The level of IFN-γ was fluctuant in the chemotherapy stage, and there was a significant difference between that in the first cycle of chemotherapy and in the second to fourth cycles of chemotherapy (P<0.05).

The level of Th1/TH2/Th17 cytokines reached a trend of stability with the relaxation of NSCLC chemotherapy. It shows that imbalance of cytokines lead and leading to immune disorder is of great significance in the diagnosis and treatment of NSCLC.