上转换荧光纳米颗粒NaYF<sub>4</sub>: Yb, Er的二氧化硅包覆及活性官能团修饰

doi:10.3877/cma.j.issn.2095-5820.2020.04.009

中华临床实验室管理电子杂志 ›› 2020, Vol. 08 ›› Issue (04) : 228 -232. doi: 10.3877/cma.j.issn.2095-5820.2020.04.009

所属专题：文献；

实验研究

上转换荧光纳米颗粒NaYF₄: Yb, Er的二氧化硅包覆及活性官能团修饰

许新强¹, 李雪松¹, 冀天星², 雷萌³, 周强²^,^†(

)

^1. 510405　广州，广州中医药大学第一附属医院　检验科
^2. 510260　广州，广州医科大学附属第二医院　检验科
^3. 336000　广东宜春，宜春市人民医院　检验科

收稿日期:2020-08-08 出版日期:2020-11-28
通信作者: 周强

Reactive functional groups decorate fluoride upconversion nanoparticles NaYF₄: Yb, Er with silica coating

Xinqiang Xu¹, Xuesong Li¹, Tianxing Ji², Meng Lei³, Qiang Zhou²^,^†()

^1. Clinical Laboratory, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, GuangZhou 510405, China
^2. Clinical Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, GuangZhou 510260, China
^3. Clinical Laboratory, Yichun People’s Hospital, Yichun Guangdong 336000, China

Received:2020-08-08 Published:2020-11-28
Corresponding author: Qiang Zhou
About author:
Corresponding author: Zhou Qiang, Email: qiangzhou70@163.com

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目的

对上转换纳米颗粒进行表面修饰，以利于生物应用。

方法

利用热分解法合成NaYF₄: Yb, Er粒子，然后采用微乳液法在粒子表面包覆一薄层SiO₂，并在其表面修饰环氧基团，采用透射电子显微镜、荧光光谱仪和傅里叶红外光谱分析仪分别对颗粒形貌、发光特性和表面性能进行表征。

结果

采用热分解法合成了大小均一，六方相的NaYF₄: Yb, Er颗粒，二氧化硅包覆纳米粒子后仍具有较强的发光特性，并进一步成功修饰上了环氧基团。

结论

上转换纳米粒子经过表面修饰后具有较强的发光特性和稳定性，可作为生物荧光标记物应用于生物医学领域。

关键词: NaYF₄: Yb, Er, 热分解法, SiO₂包覆, 环氧基团

Objective

To modify the surface of nanoparticles for biological application.

Methods

NaYF₄: Yb, Er nanoparticles were synthesized by thermal decomposition method, then a thin layer of SiO₂ was coated on the surface of above nanoparticles by microemulsion method and epoxy group was also modified on the surface. In addition, the nanoparticles morphology, luminescence properties and surface properties were characterized by transmission electron microscopy (TEM), fluorescence spectroscopy and fourier infrared spectrum analyzer(FT-IR),respectively.

Results

Uniform-size and hexagonal-phase NaYF₄: Yb, Er were synthesized by thermal decomposition method. Silica-coated NaYF₄: Yb, Er nanoparticles still had strong luminescence properties, and nanoparticles could be further modified with epoxide group.

Conclusion

The fluoride upconversion nanoparticle with strong luminescence properties and stability after surface modification could be used as a new biological fluorescent marker in the biomedical field.

Key words: NaYF₄:Yb, Er, Thermal decomposition method, Silica coated, Epoxy group

图1 β-NaYF₄: 30%Yb, 2%Er颗粒的电镜图

图2 β-NaYF₄: 30% Yb，2% Er的XRD和六角相的标准卡尺资料（JCPDS file no. 16-0034）

图3 颗粒包覆SiO₂ 的电镜图

图4 颗粒包覆SiO₂前后的荧光光谱图：a.颗粒包覆SiO₂之前的荧光光谱图　b.颗粒包覆SiO₂之后的荧光光谱图

图5 傅里叶红外光谱分析图：a. NaYF₄: 30% Yb，2% Er b. NaYF₄: 30% Yb，2% Er表面包覆SiO₂ c. NaYF₄: 30% Yb，2% Er包覆SiO₂后，并在其表面修饰环氧基

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