Comparative study of the target genes used in multiple polymerase chain reaction for the detection and discrimination of Mycobacterium

doi:10.3877/cma.j.issn.2095-5820.2016.03.007

Abstract

Abstract:

Mycobacterium is one of the most common human pathogens worldwide. There has been found more than 150 species, including M. tuberculosis complex (MTC), M. leprosy and nontuberculous mycobacteria (NTM). The MTC is the main pathogenic bacteria. There have been many reports of difficulty in differentiating closely related mycobacterial species. Therefore, molecular biological tools like polymerase chain reaction (PCR)-based assays are needed to develop for reliable and rapid detection and discrimination of MTC and NTM. PCR/multiple PCR methods targeting different genes have been used in routine diagnostic laboratories, including IS6110, 16S rRNA, 16S-23S rRNA ITS, hsp65, rpoB, gyrB and so on. Here we will review the comparative study of these targeting genes used in PCR detection methods.

Key words: Mycobacterium tuberculosis, polymerase chain reaction, Target genes, Identification

Hui Yan, Qingyi Zhu. Comparative study of the target genes used in multiple polymerase chain reaction for the detection and discrimination of Mycobacterium[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2016, 04(03): 159-163.

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