Therapeutic effect of clobetasol on white matter injury in neonatal rats

doi:10.3877/cma.j.issn.2095-5820.2023.01.002

Abstract

Abstract:

Objective

To investigate the therapeutic effects of clobetasol on premature white matter injury (WMI) in rats.

Methods

Newborn 3-day-old SD rats were selected, and the WMI model was made by ligation of the right common carotid artery and hypoxia for 80 minutes. WMI rats were intraperitoneally injected with clobetasol (2 mg·kg^-1·d^-1 and 5 mg·kg^-1·d^-1) for 5 days. The pathological damage of brain tissue in WMI rats was observed by Hematoxylin and Eeosin (HE) staining. The expression levels of myelin base protein (MBP) and CC1 were observed by immunofluorescence. The expression of iNOS was detected by Western blotting.

Results

After treated with clobetasol at 2 mg·kg^-1·d^-1 and 5 mg·kg^-1·d^-1, the loose of white matter structure was relieved, cell swelling was reduced, myelin sheath was increased, and the pathological damage of white matter was relieved. Clobetasol can promote myelination in WMI rats by up-regulating the expression levels of MBP and CC1. In addition, clobetasol alleviated myelin sheath injury in WMI rats by restoring iNOS expression.

Conclusions

Clobetasol can alleviate the pathological injury of neonatal white matter injury by increasing the expressions of MBP, CC1 and iNOS, which providing a theoretical basis for the treatment of premature WMI with clobetasol.

Key words: clobetasol, white matter injury, myelin base protein

Zhongxia Dou, Ruixia Song, Wanyu Feng, Xuewen Su. Therapeutic effect of clobetasol on white matter injury in neonatal rats[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2023, 11(01): 5-9.

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References 30

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