To analyze retrospectively flora distribution and drug resistance of deep fungus in three years of clinical isolatsed provides reasonable grounds for clinical rational utilization.

9 875 clinical samples of infected patients from outpatients and hospital wards had been retrospectively analyzed during 2012-2014 in the Third People′s Hospital of Qigndao. After conventionally being cultured and isolated, fungi were identified by VITEK-2 automatic microorganism analyzer. Drug susceptibility test was performed by the Danish Rosco antifungal susceptibility disk diffusion method to ascertain tolerance of fungi.

Six species and 237 strains of deep fungus were isolated from 9 875 clinical samples, in which the positive rate of every type candida culture was 2.4% (237/9 875). The major components of 237 deep fungus were Candida albicans and Candida tropicalis, accounted for 70.9%(168/237), 19.8%(47/237) respectively; the rest were Candida parapsilosis 2.1%(5/237), Candida lusitaniae 1.3%(3/237), Candida glabrata 2.5%(6/237) and aspergillus 3.4%(8/237). The positive rate of each reminder was 1.9%(54/2 798), 2.4%(76/3 127) and 2.7%(107/3 950), accounting for 6.9%(54/781), 8.1%(76/933) and 8.6%(107/1 248) of the entire positive samples. Moreover, the percentage of fungus culture positive samples had the tendency to increase year by year. Candida albicans and Candida tropicalis possess high resistance towards Fluconazole, Itraconazole, and Ketoconazole (Candida albicans were 16.1%(27/168), 14.3%(24/168), 14.9%(25/168); Candida tropicalis were 12.8%(6/47), 19.1%(9/47), 17.0%(8/47).

In the separation and identification of clinical specimens, deep candida detection rate increases year by year. The most isolates are Candida albicans and Candida tropicalis, with have high resistance to fluconazole, itraconazole, and ketoconazole, which need clinical attention.