Comparison of nucleic acid detection rates of three different biological samples of SARS-CoV-2 by real-time RT-PCR

doi:10.3877/cma.j.issn.2095-5820.2021.01.005

Abstract

Abstract:

Objective

To detect viral nucleic acid in nasopharyngeal swab, stool, and sputum samples of COVID-19 patients using real-time RT-PCR and compare their detection rates.

Methods

Nasopharyngeal test samples (242), stool samples (78), and sputum samples (230) were collected from COVID-19 patients at the early stage in Chengdu Public Health Clinical Medical Center, including two different biological samples at the same time collect blood from the patients (55) and at the same time to collect patients in three different biological samples (44), and the viral nucleic acids were detected by real-time RT-PCR, comparing three kinds of positive detection rate of individual and joint inspection samples.

Results

The positive detection rates of SARS-CoV-2 nucleic acid in nasopharyngeal swab samples, stool samples, and sputum samples were 32.64%, 39.74%, and 68.26%, respectively, and the differences were statistically significant (P＜0.05). The total positive rate of the combined detection of the three sample types (95.45%) was higher than that of the combined two types of samples.

Conclusion

The positive rate of the three biological samples separately was the highest in sputum detection, followed by stool samples and nasopharyngeal swabs. The combined detection of the three samples will greatly improve the detection rate of COVID-19, which provides a reference for the selection of samples in the COVID-19 laboratory test and how to improve the detection rate of nucleic acids.

Key words: Real-time RT-PCR, SARS-CoV-2, Detection rate, Sputum sample

Yi Zha, Lingyu Jiang, Ling Jian, Qingfeng Li, Zhengxiao Wei. Comparison of nucleic acid detection rates of three different biological samples of SARS-CoV-2 by real-time RT-PCR[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2021, 09(01): 24-28.

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URL: https://zhlcsysgldzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-5820.2021.01.005

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References 16

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样本类型/例数(n)	阳性例数	阴性例数	假阴性率(%)	阳性检出率(%)
鼻咽拭子(242)	79	163	67.36	32.64
粪便(78)	31	47	60.26	39.74
痰液(230)	157	73	31.74	68.26^*

样本类型/例数(n)	阳性例数	阴性例数	假阴性率(%)	阳性检出率(%)
鼻咽拭子(242)	79	163	67.36	32.64
粪便(78)	31	47	60.26	39.74
痰液(230)	157	73	31.74	68.26^*

样本类型及分组	总例数	阳性例数	阴性例数	阳性检出率（%）
样本类型及分组	总例数	阳性例数	阴性例数	单样本类型	联检
鼻咽拭子+粪便(1组)	55	42	13		76.36
鼻咽拭子		23	32	41.82
粪便		21	34	38.18
痰液+粪便(2组)	44	38	6		86.36
痰液		32	12	72.73
粪便		17	27	38.64
痰液+鼻咽拭子(3组)	55	51	4		92.73
痰液		40	15	72.73
鼻咽拭子		23	32	41.82
痰液+粪便+鼻咽拭子(4组)	44	42	2		95.45^*
痰液		32	12	72.73
粪便		17	27	38.64
鼻咽拭子		20	24	45.45

样本类型及分组	总例数	阳性例数	阴性例数	阳性检出率（%）
样本类型及分组	总例数	阳性例数	阴性例数	单样本类型	联检
鼻咽拭子+粪便(1组)	55	42	13		76.36
鼻咽拭子		23	32	41.82
粪便		21	34	38.18
痰液+粪便(2组)	44	38	6		86.36
痰液		32	12	72.73
粪便		17	27	38.64
痰液+鼻咽拭子(3组)	55	51	4		92.73
痰液		40	15	72.73
鼻咽拭子		23	32	41.82
痰液+粪便+鼻咽拭子(4组)	44	42	2		95.45^*
痰液		32	12	72.73
粪便		17	27	38.64
鼻咽拭子		20	24	45.45

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