Research and application of HBV RNA detection in monitoring the clinical cure of chronic hepatitis B patients

doi:10.3877/cma.j.issn.2095-5820.2024.02.002

Abstract

Abstract:

Objective

To investigate the role of HBV RNA detection in the treatment of chronic hepatitis B(CHB) patients.

Methods

HBV DNA and HBV RNA in serum of 56 patients with CHB were detected by real-time fluorescent quantitative PCR, and HBV surface antigen in serum of the same period was quantitatively detected by electrochemiluminescence. The Wilcoxon sign rank test was conducted on the detection values of HBV RNA and HBV DNA in 56 patients. Then, Spearman correlation analysis was made between the two values and the quantitative value of HBsAg to analyze the correlation.

Results

The serum of 56 patients with clinically diagnosis of CHB was positive for HBV RNA and HBsAg were, and 7 of them were negative for HBV DNA. The difference between HBV RNA and HBV DNA detection values was statistically significant (P<0.05). The HBV RNA and HBV DNA load were analyzed separately with HBsAg quantitation, and there was no correlation between HBV and HBsAg (all P>0.05).

Conclusions

The positive detection rate of HBV RNA and HBsAg was higher than that of HBV DNA. The positive detection of HBsAg and HBV RNA is consistent.

Key words: HBV RNA, HBV DNA, HBsAg, chronic hepatitis B

Ma Li, Ling Yan, Yun Xi, Shangxia Li, Donglin Zhu. Research and application of HBV RNA detection in monitoring the clinical cure of chronic hepatitis B patients[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2024, 12(02): 70-74.

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References 30

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项目	阳性/例	阴性/例	检出率/%
HBV DNA	49	7	87.5
HBV RNA	56	0	100.0
HBV HBsAg	56	0	100.0

项目	阳性/例	阴性/例	检出率/%
HBV DNA	49	7	87.5
HBV RNA	56	0	100.0
HBV HBsAg	56	0	100.0

项目	例数	中位数/（IU/ml）	Z值	P值
HBV DNA	56	313.500	4.870	<0.001
HBV RNA	56	4 430.000	4.870	<0.001

项目	例数	中位数/（IU/ml）	Z值	P值
HBV DNA	56	313.500	4.870	<0.001
HBV RNA	56	4 430.000	4.870	<0.001

项目	组别1		组别2
项目	例数	检测值	例数	检测值
HBV RNA	28	26 700.00（744.00，15 752 500.00）IU/ml	28	983.00（225.50，12 975.00）IU/ml
HBsAg	28	5 592.50（1 405.75，7 265.00）COI ^a	28	639.30（225.35，999.35）IU/ml ^b
r值	0.193		0.132
P值	0.326		0.504

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