目的

探讨B淋巴细胞刺激因子（B lymphocyte stimulating factor，BLyS/BAFF）及其受体在非霍奇金淋巴瘤（non-Hodgkin lymphoma，NHL）中的表达及其与NHL的关联性。

方法

采用成组配对试验诊断研究设计。用ELISA和实时荧光定量PCR测定47例NHL患者及20名健康人血清BAFF及其受体含量和外周血单个核细胞（peripheral blood mononuclear cell，PBMC）中BAFF mRNA 含量；并用配对t检验比较分析两组血清BAFF及其受体蛋白或BAFF mRNA表达水平差异，同时分析其与乳酸脱氢酶（lactate dehydrogenase，LDH）、β₂微球（β₂-microglobulin，β₂M）间的相关性。

结果

NHL组PBMC中BAFF mRNA含量（0.48±0.02）显著高于健康对照组（0.25±0.02，t=17.75，P=0.000）；NHL组血清BAFF及其受体TACI、BCMA、BAFF-R表达水平[（19.18±1.29） ng/ml、（212.74±7.98） pg/ml、（102.98±6.18） pg/ml、（2.95±0.19） ng/ml]也均较健康人明显增高[（10.94±1.56） ng/ml、（139.00±12.51） pg/ml、（59.66±7.06） pg/ml、（1.34±0.26） ng/ml，t值分别为7.05、8.61、7.99、8.66，P均＜0.01]。NHL患者中LDH升高组[（310.96±108.00）U/L]显著高于LDH正常组[（142.65±36.00） U/L]，且两组间差异有统计学意义（t=4.697，P＜0.05）。LDH升高组NHL患者血清BAFF、BCMA蛋白水平、BAFF mRNA表达水平与LDH正常组的差异均有统计学意义（t值分别为21.060，7.254，6.658；P均＜0.05）。但NHL组BAFF mRNA、血清BAFF及其受体蛋白表达水平与β₂M、LDH含量间无直线相关性（r值分别为0.026、0.014、0.127、0.001、0.003与0.201、0.093、0.015、0.031、0.169；P均＞0.05）。

结论

NHL患者PBMC中BAFF mRNA和血清BAFF及其受体蛋白表达水平均升高，BAFF及其受体可能是参与NHL发病的因素，其表达水平与疾病的活动性有关。

Objective

To measure the levels of B lymphocyte stimulating factor (BLyS/BAFF)and its receptors in non-Hodgkin's lymphoma (NHL) and to determine the relationship between BAFF and receptors level with NHL.

Methods

Serum levels of BAFF, its receptor and blood mononuclear cell(PBMC) BAFF mRNA levels of 47 NHL patients and 20 controls were determined by ELISA and real-time quantitative PCR. Paired-samples t test was used to compare the differences of serum BAFF, its receptor and BAFF mRNA levels between NHL patients and healthy controls. Meanwhile, the correlations between the expression of BAFF and its receptors and the concentration of lactate dehydrogenase (LDH), β₂-microglobulin(β₂M) were estimated.

Results

Compared with controls, NHL patients had significantly higher levels of BAFF PBMC mRNA (0.48±0.02 vs. 0.25±0.02, t=17.75, P=0.000). Serum BAFF and its receptors TACI,BCMA, BAFF-R levels in NHL patients [(19.18±1.29) ng/ml, (212.74±7.98) pg/ml, (102.98 ±6.18) pg/ml,(2.95±0.19) ng/ml] were significantly higher than those in healthy controls [(10.94±1.56) ng/ml,(139.00±12.51) pg/ml, (59.66±7.06) pg/ml, (1.34±0.26) ng/ml, t values were 7.05, 8.61, 7.99, 8.66,P＜0.01]. Compared with the normal LDH group, the elevated LDH group had significantly higher levels of LDH [(310.96±108.00) U/L vs (142.65±36.00) U/L, t=4.697, P＜0.05] , there were also significant differences in BAFF, BCMA and BAFF mRNA expression between the two groups (t values were 21.060, 7.254, 6.658, respectively, P＜0.05). However, there was no significant correlation between BAFF mRNA, serum BAFF and its receptors and β₂M, LDH (r=0.026, 0.014, 0.127, 0.001, 0.003;0.201, 0.093, 0.015, 0.031, 0.169, respectively, P＞0.05).

Conclusion

Serum BAFF, and PMBC BAFF mRNA levels are significantly elevated in NHL patients, suggesting that BAFF and its receptors may be involved in the pathogenesis of NHL and those expression may associate with the activity of NHL.