调查昆明市临床分离鉴定的75株非结核分枝杆菌分类及分析不同类别非结核分枝杆菌对抗生素的耐药性，为临床区分及治疗提供更好的依据。

选取昆明地区2017年1月至2019年9月于本院住院患者，采集患者的痰液样本，进行BD960和罗氏培养，阳性样本采用珠海银科96孔非结核微孔板药敏检测技术，在液体培养基中进行的比例法测定，根据各含药孔、对照孔的生长结果判断药物敏感性。

昆明市感染患者分离的非结核分枝杆菌主要为鸟分枝杆菌(44株)，其次为脓肿分枝杆菌(13株)、胞内分枝杆菌(10株)、龟分枝杆菌(8株)，其中以男性为主(67%)，年龄在40岁~60岁患者占比高达56%，感染系统为肺部感染占52.6%。耐药率最高的药物分别为亚胺/西司、多西环素、磺胺甲唑和头孢西丁。

昆明市非结核分枝杆菌以鸟分枝杆菌为主，主要见于肺部感染患者，不同种类的非结核分枝杆菌对抗生素耐药性各不相同，需严格密切监测其耐药情况，更好地指导临床用药。

To investigate the classification of 75 strains of non-TB mycobacteria isolated and identified in Kunming, and analyze the resistance of different types of non-TB mycobacteria to antibiotics, which provide a better basis for clinical differentiation and treatment.

Select patients admitted to the hospital from January 2017 to September 2019 in Kunming area. Sputum samples were collected and cultured by BD960 and Roche. The positive samples were detected by drug sensitivity test technique using Zhuhai silver 96-hole non-tuberculosis microporous plate. The drug sensitivity was judged according to the growth results of each drug-containing hole and the control hole.

The main non-TB mycobacteria isolated from the infected patients in Kunming were mainly bacillus (44 strains), followed by mycobacteria abscess (13 strains), intracellular mycobacteria (10 strains) and mycobacteria tortoise (8 strains). Of which male-dominated (67%), the proportion of patients aged 40 to 60 was as high as 56%, and the infection system was 52.6% lung infection. The drug with the highest rates of drug resistance were amine/sis, dosicycline, sulfonamide metformin and cephalosporin.

The main strains of non-TB mycobacteria in Kunming were bird mycobacteria, mainly found in patients with pulmonary infection. Different kinds of non-TB mycobacteria are different in their resistance to antibiotics, so it is more necessary to closely monitor the drug resistance and better guide the clinical use.