Application of capillary ultra-centrifugation in identification of Rh blood group in thalassemia patients

doi:10.3877/cma.j.issn.2095-5820.2020.04.011

Abstract

Abstract:

Objective

To investigate the application value of capillary ultra-centrifugation in the identification of Rh blood group in thalassemia patients.

Methods

Direct determination of Rh blood type and separation of autoerythrocytes by capillary hypervelocity centrifugation were performed in 49 severe thalassaemia patients who underwent repeated transfusions in our hospital. The results were compared to analyzed the value of capillary ultra-centrifugation in the separation of red blood cells in thalassemia patients.

Results

The success rate of Rh blood group identification by capillary ultracentrifugation in severe thalassaemia patients with repeated transfusions is positively correlated with the transfusion interval (r=0.844, P<0.01) . 63.26% (31/49) of severe thalassemia patients were accurately identified their Rh blood type after the separation of autoerythrocytes by capillary hypervelocity centrifugation. The proportion distribution of Rh typing results after successful separation and identification was CCDee>CcDEe>ccDEE>CCDEE.

Conclusion

The separation of autoerythrocytes by capillary hypervelocity centrifugation was helpful for the identification of Rh blood type in thalassemia patients with repeated transfusion, providing the basis for their Rh matching transfution and reducing homotypic immune response.

Key words: Capillary ultracentrifugation, Thalassemia, Rh blood group identificatio, Transfusion

Yuncong Zhang, Wenqing Huang, Junjie Wei, Tianzi Guo, Shiqiao Zhou. Application of capillary ultra-centrifugation in identification of Rh blood group in thalassemia patients[J]. Chinese Journal of Clinical Laboratory Management(Electronic Edition), 2020, 08(04): 237-240.

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References 18

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Rh抗原	例数	原始样本结果			毛细管分离后
Rh抗原	例数	阳性	阴性	双群	阳性	阴性	双群	双群下降率（%）
C	49	43	1	5	44	2	3	4.08
c	49	9	3	37	17	16	16	42.86
D	49	48	1	0	48	1	0	0.00
E	49	7	5	37	16	19	14	46.94
e	49	43	1	5	45	2	2	6.12
合计	245	150	11	84	170	40	35

Rh抗原	例数	原始样本结果			毛细管分离后
Rh抗原	例数	阳性	阴性	双群	阳性	阴性	双群	双群下降率（%）
C	49	43	1	5	44	2	3	4.08
c	49	9	3	37	17	16	16	42.86
D	49	48	1	0	48	1	0	0.00
E	49	7	5	37	16	19	14	46.94
e	49	43	1	5	45	2	2	6.12
合计	245	150	11	84	170	40	35

Rh表型	例数	占比（%）
CCDee	13	41.94
CcDEe	11	35.48
CcDee	2	6.45
CCDEe	2	6.45
ccDEE	2	6.45
CcDee	1	3.23
合计	31

Rh表型	例数	占比（%）
CCDee	13	41.94
CcDEe	11	35.48
CcDee	2	6.45
CCDEe	2	6.45
ccDEE	2	6.45
CcDee	1	3.23
合计	31

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